Results 91 to 100 of about 36,597 (195)

Pathways of genetic adaptation: multistep origin of mutants under selection without induced mutagenesis in Salmonella enterica. [PDF]

, 2012
In several bacterial systems, mutant cell populations plated on growth-restricting medium give rise to revertant colonies that accumulate over several days.
Quiñones-Soto, Semarhy, Reams, Andrew B, Roth, John R   +2 more
core  

Pseudomonas aeruginosa Biofilms in Cystic Fibrosis: Interactions, Methods, and Therapeutic Strategies

BioMed Research International, Volume 2026, Issue 1, 2026.
This review explores the role of Pseudomonas aeruginosa biofilms in cystic fibrosis (CF) pathogenesis. Biofilms, the main bacterial lifestyle in CF lungs, are key in therapy failure, immune evasion, and chronic infection persistence. This review examines biofilm structure, emphasizing extracellular polymeric substances (Psl, Pel, alginate, eDNA) and ...
Luis Ángel Núñez-García, Carlos Córdova-Fletes, María Carmen Barboza-Cerda, Elvira Garza-González, Baisakhi Banerjee   +4 more
wiley   +1 more source

A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli

Scientific Reports
Protein production using Escherichia coli is a cornerstone of modern biotechnology. In this study, we developed a novel auto-expression medium to maximize protein production. Each E.
James Bosco, Emily Gagliano, Kassandra L. Boshae, John P. Statz, Timothy B. Wheeler, DeAnna Cuello, Ashlyn Sliter, Christian Newby, Bernice Lin, Aysha Demeler, C. Logan Pierpont, Cindee Yates-Hansen, Matthew J. Sydor, Maria E. Ferrini, Kellie C. Kuch, Brandon S. Cooper, Beverly J. Piggott, Sarah J. Certel, Kasper B. Hansen, Stephen R. Sprang, Bruce Bowler, Levi McClelland, Mehmet Berkmen, Ekaterina Voronina   +23 more
doaj   +1 more source

Exploiting prokaryotic chitin-binding proteins for glycan recognition [PDF]

, 2011
• The cloning, expression and characterisation of prokaryotic chitin-binding proteins from Serratia marcescens, Pseudomonas aeruginosa, Photorhabdus luminescens Microfluidics and Photorhabdus asymbiotica • Development of an assay to assess the activity
Clarke, Paul A., Larragy, Ruth, O'Connell, Michael, O'Connor, Brendan, Thompson, Roisin   +4 more
core  

In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins

ChemBioChem, Volume 26, Issue 22, November 17, 2025.
This review evaluates engineered (semi)autonomous cell systems for the biosynthesis and incorporation of noncanonical amino acids (ncAAs) into proteins. While semi‐autonomous cells convert supplied precursors into ncAAs autonomous cells integrate biosynthetic pathways that produce these building blocks intracellularly.
Jan Hendrik Illies, Tim Moritz Weber, Ivana Drienovská   +2 more
wiley   +1 more source

Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

Microbial Cell Factories, 2020
Background The genome-integrated T7 expression system offers significant advantages, in terms of productivity and product quality, even when expressing the gene of interest (GOI) from a single copy.
Artur Schuller, Monika Cserjan-Puschmann, Christopher Tauer, Johanna Jarmer, Martin Wagenknecht, Daniela Reinisch, Reingard Grabherr, Gerald Striedner   +7 more
doaj   +1 more source

Genetic Surfaceome E. coli Reprogramming Enables Selective Water Oxidation

Advanced Materials, Volume 37, Issue 47, November 27, 2025.
A novel enzymatic material based on bilirubin oxidase (BOD) surface‐expressed on E. coli for effective electrocatalysis is reported. With this material, highly efficient and selective water oxidation is achieved, a critical half reaction for synthetic photosynthesis. Abstract Programming catalytic behavior at the microbial genome level is a frontier in
Graziela C. Sedenho, Jéssica C. Pacheco, Melanie Gut, Filipe C. D. A. Lima, Sunanda Dey, Frank N. Crespilho, Ariel L. Furst   +6 more
wiley   +1 more source

Regulation of endothelial-specific transgene expression by the LacI repressor protein in vivo.

PLoS ONE, 2014
Genetically modified mice have played an important part in elucidating gene function in vivo. However, conclusions from transgenic studies may be compromised by complications arising from the site of transgene integration into the genome and, in ...
Susan K Morton, Daniel J Chaston, Brett K Baillie, Caryl E Hill, Klaus I Matthaei   +4 more
doaj   +1 more source

Corrigendum: The selective advantage of the lac operon for Escherichia coli is conditional on diet and microbiota composition. [PDF]

Front Microbiol, 2023
Pinto C, Melo-Miranda R, Gordo I, Sousa A.   +3 more
europepmc   +1 more source

Historical Contingency Causes Divergence in Adaptive Expression of the lac Operon. [PDF]

Mol Biol Evol, 2021
Karkare K, Lai HY, Azevedo RBR, Cooper TF.   +3 more
europepmc   +1 more source