Results 81 to 90 of about 2,070,930 (266)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

O pacote fiscal de 1995: estratégias que otimizam os recursos dos investidores

RAE: Revista de Administração de Empresas, 1995
Uma estratégia a ser utilizada pelos gestores após a modificação tributária da Lei nº 8.981/95, que aumentou substancialmente os impostos sobre lucros e limitou a utilização dos prejuízos fiscais acumulados.
Jairo Laser Procianoy, Francisco O. V. Schmitt   +1 more
doaj  

High-power optical amplifier with enhanced wall-plug efficiency for 10-channel WDM satellite laser communication systems [PDF]

EPJ Web of Conferences, 2022
Hochheim Sven, Büttner Alexander, Brockmüller Eike, Fittkau Willy, Wellmann Felix, Weßels Peter, Neumann Jörg, Kracht Dietmar   +7 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Towards ultra fast pulse generation by gain-switching of diode pumped surface emitting semiconductor lasers [PDF]

EPJ Web of Conferences, 2022
Marianovich André, Spiekermann Stefan, Brendel Moritz, Weßels Peter, Neumann Jörg, Weyers Markus, Kracht Dietmar   +6 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Inovação tecnológica: a privatização após o uso das "moedas podres"

RAE: Revista de Administração de Empresas, 1992
Após uma análise dos modelos econômicos de privatização e dos instrumentos financeiros inovadores, apresentamos os aspectos relevantes das Ações Preferenciais Resgatáveis (APR) brasileiras, como uma forma de obter recursos junto ao mercado de capitais e ...
Jairo Laser Procianoy, Paulo Cesar Delayti Motta   +1 more
doaj  

O uso de informações por analistas de investimento na avaliação de empresas: à procura de padrões

RAC: Revista de Administração Contemporânea
A informação é hoje um dos principais recursos dos trabalhadores do conhecimento na resolução de problemas, mas pouco se sabe sobre a natureza de sua utilização.
Luiz Henrique Boff, Jairo Laser Procianoy, Norberto Hoppen   +2 more
doaj   +1 more source

Forage characteristics of Vicia sativa L. and Trifolium resupinatum L. in catch crop systems under Central European conditions

Plant, Soil and Environment, 2005
In a field experiment the development of dry matter yield and forage quality of the catch crop Vicia sativa L. compared with Trifolium resupinatum L. was investigated and the suitability for cultivation in a mixture with Lolium multiflorum ssp. gaudinii (
W. Opitz Von Boberfeld, E. Beckmann, H. Laser   +2 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source