The polarization direction-dependent SBS threshold was investigated, and the terminal polarization control technique was demonstrated to restore the linear polarization state.
Yu Wen +9 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Excimer laser treatment of ZE41 magnesium alloy for corrosion resistance and microhardness improvement [PDF]
We would like to acknowledge the financial support of the ‘‘Conseil regional de PACA’’ and Protection des Metaux d’Arenc). The authors are grateful to PMA’s engineers Ms. E. Castellan, Ms. A. Gonthier and Mr. F.
KHALFAOUI, Walid +3 more
core +1 more source
Threonine 348 regulates the subcellular localization of PTEN
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley +1 more source
Laser powder bed fusion of glass: A comparative study between CO2 lasers and ultrashort laser pulses
Art. 112710T, 6 S.During the last decade, laser assisted additive manufacturing evolved to a serious alternative to traditional manufacturing methods. The greatest benefit lies in the realization of almost any desired geometry impossible to create with ...
Seyfarth, B. +6 more
core +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
Influence of various process conditions on surface finishes induced by the direct metal deposition laser technique on a Ti–6Al–4V alloy [PDF]
The direct metal deposition (DMD) with laser is a free-form metal deposition process for manufacturing dense pieces, which allows generating a prototype or small series of near net-shape structures. One of the most critical issues is that produced pieces
GORNY, Cyril +7 more
core +1 more source
Four-Channel Nanosecond Pulse Combination in the Non-Polarization-Maintaining Fiber System
We report a novel coherent nanosecond pulse combination approach using four-channel non-polarization-maintaining large-mode-area (LMA) Ytterbium-doped (Yb-doped) fiber amplifiers.
Xinyu Wang +10 more
doaj +1 more source
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source

