Results 31 to 40 of about 72,360 (255)

Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angle [PDF]

Microscopy Research and Technique, 2006
AbstractLaser illumination used in both conventional widefield epi‐fluorescence as well as in total internal reflection fluorescence (TIRF) microscopy is subject to nonuniformities in intensity that obscure true image details. These intensity variations are interference fringes arising from coherent light scattering and diffraction at every surface in ...
Mattheyses, Alexa L., Shaw, Keith D., Axelrod, Daniel   +2 more
openaire   +3 more sources

Evaluation of nonlinear dynamics of the cell structure damage as a promising method for personalized cancer diagnostics

Alʹmanah Kliničeskoj Mediciny, 2018
Background: Modulation interference microscopy is one of the promising technologies for early personalized cancer diagnostics, while it gives the possibility to obtain real-time images of living functioning cells at a nanometer resolution, as well as to ...
O. B. Naimark, A. S. Nikityuk, V. O. Nebogatikov, V. V. Grishko   +3 more
doaj   +1 more source

Improving the homogeneity of diffraction based colours by fabricating periodic patterns with gradient spatial period using Direct Laser Interference Patterning

Scientific Reports, 2019
This study focuses on the development of a strategy to produce periodic structures with a variable spatial period for increasing the homogeneity of structural colours by means of direct laser interference patterning.
Bogdan Voisiat, Wei Wang, Max Holzhey, Andrés Fabián Lasagni   +3 more
doaj   +1 more source

Application of Laser Feedback Interference Microscopy to Localize the Ciliary Vector

The FASEB Journal, 2015
In migrating fibroblasts with primary cilia, the primary cilium points in the direction of migration but why this is so is unknown. The objective of our work is to investigate whether directional signaling from the primary cilium (the ciliary vector) affects the direction of cell migration or vice versa and to determine what molecules and mechanisms ...
Ben Ovryn, Peter Satir
openaire   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Hierarchical Micro-/Nano-Structures on Polycarbonate via UV Pulsed Laser Processing

Nanomaterials, 2020
Hierarchical micro/-nanostructures were produced on polycarbonate polymer surfaces by employing a two-step UV-laser processing strategy based on the combination of Direct Laser Interference Patterning (DLIP) of gratings and pillars on the microscale (3 ...
Marek Mezera, Sabri Alamri, Ward A.P.M. Hendriks, Andreas Hertwig, Anna Maria Elert, Jörn Bonse, Tim Kunze, Andrés Fabián Lasagni, Gert-willem R.B.E. Römer   +8 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Coherent scatter-controlled phase-change grating structures in silicon using femtosecond laser pulses

Scientific Reports, 2017
Periodic structures of alternating amorphous-crystalline fringes have been fabricated in silicon using repetitive femtosecond laser exposure (800 nm wavelength and 120 fs duration). The method is based on the interference of the incident laser light with
Yasser Fuentes-Edfuf, Mario Garcia-Lechuga, Daniel Puerto, Camilo Florian, Adianez Garcia-Leis, Santiago Sanchez-Cortes, Javier Solis, Jan Siegel   +7 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Bloch Surface Wave Assisted Structured Illumination Microscopy for Sub-100 nm Resolution

IEEE Photonics Journal, 2021
Structured illumination microscopy (SIM) is one of the most powerful and versatile super-resolution methods due to its live-cell imaging ability of subcellular structures with high speed.
Weijie Kong, Changtao Wang, Mingbo Pu, Xiaoliang Ma, Xiong Li, Xiangang Luo   +5 more
doaj   +1 more source