Results 31 to 40 of about 287,305 (267)

Evaluation of finite element simulation of water quenched cracking for medium carbon alloy steels using acoustic emission technique

Journal of Materials Research and Technology, 2023
Quenching is an important technology for enhancing strength of steels. It is the development tendency of heat treatment to replace both polluted oil quenching and polymer aqueous solution quenching with clean water quenching. However, the problem of easy
Jie Li, Liyang Zeng, Shuai Wang, Xiangyu Song, Nailu Chen, Xunwei Zuo, Yonghua Rong   +6 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Microscopic strain localisation in WAAM Ti-6Al-4V during uniaxial tensile loading [PDF]

MATEC Web of Conferences, 2020
Wire Arc-Based Additive manufacturing is a high deposition rate process suitable for building large-scale aerospace components. However, the larger heat source can cause greater microstructural heterogeneity and, in particular, a coarse columnar ß grain ...
Lunt David, Ho Alistair, Davis Alec, Martina Filomeno, Hönnige Jan, Quinta da Fonseca João, Prangnell Phillip   +6 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Near-infrared laser interference patterning of silver nanocomposite films: controlling spatial nanoparticle distributions for plasmonic coloring

JPhys Photonics
Nanocomposites formed by metal nanoparticles embedded in dielectric host matrices present exciting optical properties governed mostly by the material, size, shape and separation of the nanoparticles. This opens the opportunity to drastically change their
José Gonzalo, Mario Garcia-Lechuga, Giorgio Baraldi, Alka Philip, Jan Siegel   +4 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Monitoring of the Weld Pool, Keyhole Morphology and Material Penetration State in Near-Infrared and Blue Composite Laser Welding of Magnesium Alloy

Journal of Manufacturing and Materials Processing
The laser welding of magnesium alloys presents challenges attributed to their low laser-absorbing efficiency, resulting in instabilities during the welding process and substandard welding quality.
Wei Wei, Yang Liu, Haolin Deng, Zhilin Wei, Tingshuang Wang, Guangxian Li   +5 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source