Results 61 to 70 of about 287,305 (267)

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Biomimetic surface structures in steel fabricated with femtosecond laser pulses: influence of laser rescanning on morphology and wettability

Beilstein Journal of Nanotechnology, 2018
The replication of complex structures found in nature represents an enormous challenge even for advanced fabrication techniques, such as laser processing.
Camilo Florian Baron, Alexandros Mimidis, Daniel Puerto, Evangelos Skoulas, Emmanuel Stratakis, Javier Solis, Jan Siegel   +6 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Revealing the Enhancement Mechanism of Laser Cutting on the Strength–Ductility Combination in Low Carbon Steel

Metals
The strength–ductility mechanism of the low-carbon steels processed by laser cutting is investigated in this paper. A typical gradient-phased structure can be obtained near the laser cutting surface, which consists of a laser-remelted layer (LRL, with ...
Jie Chen, Feiyue Tu, Pengfei Wang, Yu Cao   +3 more
doaj   +1 more source

Cracking mechanism and susceptibility of laser melting deposited Inconel 738 superalloy

Materials & Design, 2019
Laser Melting Deposition (LMD) of Inconel 738 (IN738) superalloy is a promising process for the remanufacturing of gas turbines and aerospace engines, but the cracking has not been thoroughly understood and controlled. This paper conducts a comprehensive
Xiaoqiang Zhang, Huabin Chen, Luming Xu, Jijin Xu, Xukai Ren, Xiaoqi Chen   +5 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Random laser emission at dual wavelengths in a donor-acceptor dye mixture solution

Results in Physics, 2017
The work was aimed to generate random laser emissions simultaneously at two wavelengths in a weakly scattering system containing mixture of binary dyes, rhodamine-B (Rh-B) and oxazine-170 (O-170) dispersed with ZnO nano-particles serving as scattering ...
Sunita Kedia, Sucharita Sinha
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source