Results 51 to 60 of about 223,812 (206)

Lunar Laser Ranging Retroreflectors: Velocity Aberration and Diffraction Pattern

open access: yesThe Planetary Science Journal, 2023
The Lunar Laser Ranging (LLR) retroreflector arrays have been on the Moon for half a century. During that time, the laser range uncertainty has improved by a factor of 100. Consequently, the science results have also improved by orders of magnitude.
James G. Williams   +9 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A Laser Ranging System Using Chaotic Light

open access: yesJournal of Measurement Science and Instrumentation, 2011
A novel laser ranging system using chaotic laser as probe light source is designed and presented.The system is made up of five components:chaotic light source,transmitter,receiver,data acquisition unit and data processing unit.Chaotic light is generated ...
Tong ZHAO   +3 more
doaj  

Research on Large Divergence Angle Laser Ranging System

open access: yesPhotonics
This study investigates a laser ranging technology scheme featuring a large divergence angle for both the emitted and received laser beams, focusing on applications where both the measured target and the ranging carrier are high-mobility platforms.
Junwen Ji, Suhui Yang, Yimin Feng
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Rapid Laser Ranging Method for Compact LiDAR Systems

open access: yesRemote Sensing
We propose a rapid laser ranging method for compact LiDAR systems. It employs a high signal-to-noise ratio (SNR) coherent detection scheme to minimize the required measurement integration time, while also addressing key challenges encountered by existing
Jiahui Guo   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

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