Results 51 to 60 of about 3,239 (163)

Research on laser measuring device of surface displacements of mine roadway

Gong-kuang zidonghua, 2018
In view of problems of inconvenient measurement, time consuming, high labor intensity and uncontrollable errors of existing cross-measuring methods of surface displacements of mine roadway, a laser measuring device of surface displacements of mine ...
YE Meitu, LIANG Yiwei, WANG Kun
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

GNSS Real-Time ZTD/PWV Retrieval Based on PPP with Broadcast Ephemerides

Atmosphere
GNSS precise point positioning (PPP) plays an important role in retrieving atmospheric water vapor values and performing numerical weather prediction. However, traditional PPP relies on real-time orbits and clocks, which require continuous internet or ...
Zongqiu Xu, Shuhao Liu, Yantian Xu, Longjiang Tang, Nannan Yang, Gen Zhang   +5 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

MOONLIGHT: A NEW LUNAR LASER RANGING RETROREFLECTOR INSTRUMENT

Acta Polytechnica, 2013
Since 1969 Lunar Laser Ranging (LLR) to the Apollo Cube Corner Reflector (CCR) arrays has supplied several significant tests of gravity: Geodetic Precession, the Strong and Weak Equivalence Principle (SEP, WEP), the Parametrized Post Newtonian (PPN ...
M. Garattini, S. Dell’Agnello, D. Currie, G. O. Delle Monache, M. Tibuzzi, G. Patrizi, S. Berardi, A. Boni, C. Cantone, N. Itaglietta, C. Lops, M. Maiello, M. Martini, R. Vittori, G. Bianco, R. March, G. Bellettini, R. Tauraso   +17 more
doaj   +1 more source

Design and Development of High-Repetition-Rate Satellite Laser Ranging System [PDF]

Journal of Astronomy and Space Sciences, 2015
The Accurate Ranging System for Geodetic Observation – Mobile (ARGO-M) was successfully developed as the first Korean mobile Satellite Laser Ranging (SLR) system in 2012, and has joined in the International Laser Ranging Service (ILRS) tracking network,
Eun-Jung Choi, Seong-Cheol Bang, Ki-Pyoung Sung, Hyung-Chul Lim, Chan-Gyu Jung, In-Yeung Kim, Jae-Seung Choi   +6 more
doaj   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source