Results 71 to 80 of about 1,362,475 (304)

Estimation of 3D vegetation structure from waveform and discrete return airborne laser scanning data [PDF]

open access: yes, 2012
This study presents and compares new methods to describe the 3D canopy structure with Airborne Laser Scanning (ALS) waveform data as well as ALS point data.
Olofsson, Kenneth,   +12 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Sub-canopy terrain modelling for archaeological prospecting in forested areas through multiple-echo discrete-pulse laser ranging: a case study from Chopwell Wood, Tyne & Wear [PDF]

open access: yes, 2011
Airborne Light Detection and Ranging (LiDAR) technology is assessed for its effectiveness as a tool for measuring terrain under forest canopy. To evaluate the capability of multiple-return discrete-pulse airborne laser ranging for detecting and resolving
LANDY, JACK
core  

Simulation of laser-driven cratering experiments on aluminum [PDF]

open access: yes, 2019
After a brief description of the physical principles involved in the cratering process, the authors present a specific methodology to simulate laser-driven cratering experiments performed with a long pulse duration (100 ns) and a small focal spot ...
Jean-Luc Rullier   +11 more
core   +1 more source

A Deformed Shape Monitoring Model for Building Structures Based on a 2D Laser Scanner

open access: yesSensors, 2013
High-rise buildings subjected to lateral loads such as wind and earthquake loads must be checked not to exceed the limits on the maximum lateral displacement or the maximum inter-story drift ratios. In this paper, a sensing model for deformed shapes of a
Hyo Seon Park   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

open access: yesFEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Applicability of 3D laser scanning in precision horticulture

open access: yesInternational Journal of Horticultural Science, 2011
Due to the technological development, remote sensing instruments and methods have become widespread in all segments of life (from precision agriculture through architecture to medicine).
J. Tamás   +6 more
doaj   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

open access: yesFEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima   +8 more
wiley   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

open access: yesFEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

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