Results 11 to 20 of about 255,657 (229)
Optimizing photoactivation of PA-mCherry for optical pooled CRISPR screens. [PDF]
FEBS Open BioPhotoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Mukherjee S +3 more
europepmc +2 more sources
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells [PDF]
FEBS Open BioMicroscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Hink M.
europepmc +2 more sources
Resolution in the ApoTome and the confocal laser scanning microscope: comparison [PDF]
Journal of Biomedical Optics, 2009 The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens.
Weigel, Arwed +2 more
openaire +3 more sources
The Transmission Confocal Laser Scanning Microscope [PDF]
Microscopy Today, 1992 Abstract A confocal laser scanning microscope which can collect images in both transmission and reflection modes has been installed and is being tested in the Imaging Laboratories of the John P. Roberts Research Institute, London, Ontario. Designed by Dr.
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Microscopic Spectrophotometry Using Confocal Laser Scanning Microscope
BUNSEKI KAGAKU, 2008 共焦点レーザー走査型顕微鏡（CLSM）を用いた顕微吸光度分析の精度及び適応範囲などを検証するために，粒径が50及び150 μmの2種類の球状シリカゲルにジヒドロオキソ（テトラフェニルポルフィリナト）アンチモン臭化物錯体（1）を0.70～3.59 mmol dm－3のモル濃度（C1m）で吸着させてマイクロビーズ（2）を調製した．CLSMでの試料の測定領域は，対物レンズの倍率及びピンホールの直径又はファイバーコアの直径によって決められる直径が2.14 μmの円形内であることが分かった．更に分析範囲，測定範囲などの分析条件を検討した結果，4.9～350 μmの球状試料について測定波長が488～775 nmで分析可能であることが分かった．そこで ...
Matsumoto, Tomoko +4 more
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Enhanced scanning control in a confocal scanning laser microscope [PDF]
Scanning, 1996 AbstractA fast and flexible scanning unit, allowing scanning rates of more than 1 kHz over regions identified in a specimen, has been developed and evaluated. This scanning unit replaces the original scanning unit in the Phoibos confocal scanning laser microscope and features full backward compatibility, while at the same time allowing fast and ...
openaire +2 more sources
Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells
FEBS Letters, EarlyView.DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
An optical spectrometer for a confocal scanning laser microscope [PDF]
Scanning, 1995 AbstractAn optical spectrometer for the visible range has been developed for the confocal scanning laser microscope (CSLM) Phoibos 1000. The spectrometer records information from a single point or a user‐defined region within the microscope specimen. A prism disperses the spectral components of the recorded light over a linear CCD photodiode array with
openaire +1 more source