Results 51 to 60 of about 379,616 (340)

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Potential of confocal laser scanning microscopy for non-invasive diagnostics of malignant epithelial skin tumors in the course of dermatoheliosis progression

Vestnik Dermatologii i Venerologii, 2017
Most cases of malignant epithelial skin neoplasms including actinic keratosis and basal cell carcinoma, which are characterized by the most complicated course and numerous clinical and morphological options, involve dermatoheliosis progression.
E. S. Snarskaya, S. B. Tkachenko, E. V. Kuznetsova, A. S. Allenova   +3 more
doaj   +1 more source

A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell [PDF]

, 2009
A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and shape of the ...
Aillaud, A., Ferrand, P., Kress, A., Marguet, D., Pianta, M., Rigneault, H.   +5 more
core   +2 more sources

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Microscopy study of ripples created on steel surface by use of ultra short laser pulses [PDF]

, 2010
This paper concentrates on ripples on the surface of steel that arise from lasermaterial interaction. In particular we have observed two different sets of ripples on steel samples that were machined by 210 fs laser pulses with 800 nm wavelength at normal
Hosson, J.Th.M. de, Huis in 't Veld, A.J., Mitko, V.S., Ocelik, V., Römer, G.R.B.E., Skolski, J.Z.P., Vincenc Obona, J., Visser, B.   +7 more
core   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Coherent microscopy and optical coherence tomography for biomedical applications [PDF]

, 2011
This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece.
3rd Micro and Nano Flows Conference (MNF2011), Coupland, JM   +1 more
core  

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy

Bio-Protocol, 2018
Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display
Natalia Cattelan, Osvaldo Yantorno, Rajendar Deora   +2 more
doaj   +1 more source

Instantaneous Clearing of Biofilm (iCBiofilm): an optical approach to revisit bacterial and fungal biofilm imaging

Communications Biology, 2023
A new clearing and imaging technique termed iCBiofilm is developed for imaging whole bacterial and fungal biofilms using confocal laser scanning microscopy, which enables examining the dynamics and functions of biofilms.
Shinya Sugimoto, Yuki Kinjo
doaj   +1 more source