Results 61 to 70 of about 1,533,904 (293)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Determination of Laser Tracker Angle Encoder Errors

open access: yesEPJ Web of Conferences, 2014
Errors in the angle encoders of a laser tracker may potentially produce large errors in long range coordinate measurements. To determine the azimuth angle encoder errors and verify their values stored in the tracker’s internal error map, several ...
Nasr Karim M.   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

New Method of Tooth Pitch Deviation Measurement based on Laser Tracker for Mega Spur Gear

open access: yesJixie chuandong, 2019
To measure pitch deviation of mega spur gears, a new method for measuring the pitch of mega spur gears based on laser tracker is proposed. The measuring ability of laser tracker in large space is used to measure gear groove, and two adjacent pitch error ...
Zhang Bai, Lin Jiachun
doaj  

Impedance Control Self-Calibration of a Collaborative Robot Using Kinematic Coupling

open access: yesRobotics, 2019
This paper presents a closed-loop calibration approach using impedance control. The process is managed by a data communication architecture based on open-source tools and designed for adaptability.
Nicholas A. Nadeau   +2 more
doaj   +1 more source

Uporaba AIFM-tehnologije za dimenzijsko kontrolo turbinskih delov ( = Use of AIFM technology in dymensional control of turbin parts) [PDF]

open access: yesGeodetski Vestnik, 2014
Conventional measuring methods in a dimensional control of turbine parts are demanding and time-consuming, their accuracy depends on experiences of measuring team.
Iztok Slatinšek, Dušan Kogoj
doaj   +1 more source

Measurement of inertia and center of gravity using laser tracker [PDF]

open access: yes, 2019
Tato bakalářská práce se zabývá odvozením a následným experimentem v oblasti dynamiky tuhého tělesa. Zabývá se zjišťováním polohy těžiště a matice setrvačnosti pomocí Laser Trackeru.This diploma is about experimental defining of center of mass and ...
Tomáš Šantrůček
core  

Construction of the CMS Tracker

open access: yes, 2007
The CMS Tracker is the largest tracker ever built entirely based on silicon detectors. Surrounding the innermost pixel system, strip detectors populate the radial region between 20 and 110 cm, for a total active surface of and almost 10 million readout ...
Hartmann, F   +10 more
core   +1 more source

The CMS Tracker Alignment Strategy [PDF]

open access: yes, 2006
CMS silicon Tracker alignment consists of three key components: Survey during tracker construction, measurements with the Laser Alignment System during operation and track based alignment. Methods and results are explained in detail, with a special focus
Martin Weber, Weber, Martin
core   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

open access: yesFEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer   +7 more
wiley   +1 more source

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