Results 41 to 50 of about 1,468,079 (289)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

FEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés, Luise Franz, Christian P.R. Hackenberger   +2 more
wiley   +1 more source

Extracellular vesicle lipidomics liquid biopsy reveals systemic and tumor microenvironment metabolic reprogramming in ovarian cancer

Molecular Oncology, EarlyView.
Extracellular vesicle (EV) lipidomic profiling of plasma and ascites from patients with high‐grade serous ovarian cancer and of ovarian cancer cell lines reveals enrichment of triglycerides (TGs), diacylglycerols (DGs), phosphatidylcholine (PCs), sphingomyelins (SMs), reflecting tumor metabolic reprogramming.
Shikha Rani, Dominic Guanzon, Andrew Lai, Katherin Scholz‐Romero, Sarah Reed, Edita Ritmejeryte, Rohan Lourie, Naven Chetty, Kaltin Ferguson, Carlos Salomon   +9 more
wiley   +1 more source

Robust estimation method for raw observations from laser tracker

AIP Advances
The triangulateration network adjustment method is a fundamental algorithm for constructing a measurement system composed of multiple laser trackers. This method is based on a prior-weight-matrix model considering varied accuracies of angle and distance ...
Yixin Ma, Weihu Zhou, Zili Zhang, Dengfeng Dong, Xiaoyan Shen, Fanchang Meng, Yang Li, Shan Wang   +7 more
doaj   +1 more source

Evaluation of binocular eye trackers and algorithms for 3D gaze interaction in virtual reality environments [PDF]

, 2008
Pfeiffer T, Latoschik ME, Wachsmuth I. Evaluation of binocular eye trackers and algorithms for 3D gaze interaction in virtual reality environments. JVRB - Journal of Virtual Reality and Broadcasting.
Latoschik, Marc Erich, Pfeiffer, Thies ; https://orcid.org/, Wachsmuth, Ipke ; https://orcid.org/   +2 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Study on LT Accuracy Improvement by Calibration Based on Network Measurements

Sensors, 2021
Laser trackers (LT) are widely used to calibrate other machines. Nevertheless, very little is known about calibrating an LT. There are some standards that allow us to evaluate the LT performance. However, they require specialized equipment. A calibration
Jesús Velázquez, Javier Conte, Ana Cristina Majarena, Jorge Santolaria   +3 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Multi-layer Rotation Memory Model-based correlation filter for visual tracking

Frontiers in Physics, 2022
Object tracking technology is of great significance in laser image processing. However, occlusion or similar interference during visual object tracking may reduce the tracking precision or even cause tracking failure.
Yufei Zhao, Yong Song, Yong Song, Guoqi Li, Lei Deng, Yashuo Bai, Yashuo Bai, Xiyan Wu, Xiyan Wu   +8 more
doaj   +1 more source

Design of a cost-effective laser spot tracker

SPIE Proceedings, 2017
One of the most important aspects of guided systems is detection. The most convenient detection in the sense of precision can be achieved with a laser spot tracker. This study deals with a military grade, high performance and cost-effective laser spot tracker for a guided system.
Artan, Goktug Gencehan, Sari, Huseyin
openaire   +2 more sources