Results 51 to 60 of about 1,468,079 (289)

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Modelling and optimization of a modified sequential multilateration method for three-dimensional coordinate collection

Advances in Mechanical Engineering, 2019
This article proposes a modified sequential multilateration method for measuring three-dimensional coordinates. The measuring system consists of a single laser tracker and four relay targets whose relative positions have been pre-calibrated by the ...
Zhenjiu Zhang, Mingjun Liu, Jiaji Jiang, Linzhong Xia, Xiaomei Xu, Wei Zhang, Zhenyu Zhao   +6 more
doaj   +1 more source

Development of a Submillimetric GNSS-Based Distance Meter for Length Metrology

Sensors, 2021
Absolute distance determination in the open air with an uncertainty of a few tenths of a millimetre is increasingly required in many applications that involve high precision geodetic metrology.
Luis García-Asenjo, Sergio Baselga, Chris Atkins, Pascual Garrigues   +3 more
doaj   +1 more source

2D longitudinal modeling of heat transfer and fluid flow during multilayered [PDF]

, 2012
version post-print de l'article : JLA Vol : 24 Iss:3. 2D longitudinal modeling of heat transfer and fluid flow during multilayered direct laser metal deposition processDerived from laser cladding, the Direct Laser Metal Deposition (DLMD) process is ...
CARRON, Denis, LE MASSON, Philippe, GHARBI, Myriam, PEYRE, Patrice, FABBRO, Rémy, CARIN, Muriel, MORVILLE, Simon   +6 more
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A Transit Tilt and Offset Errors Calibration Method for Improving Laser Tracker Measurement Accuracy Based on the Telecentric Measurement System

Applied Sciences
Laser trackers are instruments used to measure the three-dimensional coordinates of objects with high precision. It is necessary to study calibration methods to identify geometric errors of laser trackers so as to compensate for errors and improve ...
Shan Wang, Zili Zhang, Dengfeng Dong, Tianci Feng, Qifan Qiu, Fanchang Meng, Guoming Wang, Chengjun Cui, Rongyi Ji, Yingling Pan, Tao Gong, Weihu Zhou   +11 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source