Results 221 to 230 of about 345,157 (301)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

Integrated Transcriptomic and Proteomic Analyses Revealed the Mechanism of the Osmotic Stress Response in <i>Lacticaseibacillus rhamnosus</i> ATCC 53103. [PDF]

Foods
Luo W, He X, Chen Y, Xu Y, Zhuang Y, Ding Y, Fan X.   +6 more
europepmc   +1 more source

A Testis‐Specific Aralkylamine N‐Acetyltransferase Regulates Dimorphic Sperm Function and Male Fertility in Moths

Advanced Science, EarlyView.
We identify a Lepidoptera‐conserved testis‐specific arylalkylamine N‐acetyltransferase (LTNAT) that governs male moth fertility via a novel mechanism. LTNAT loss disrupts eupyrene sperm mitochondrial derivatives and impairs apyrene sperm motility, offering a safe molecular target for innovative pesticides and genetic pest control.
Hao Sun, Peng‐Yi Huang, Zhi‐Ruo Zhang, Cong‐Fen Gao, Subba Reddy Palli, Shun‐Fan Wu   +5 more
wiley   +1 more source

A Novel Approach to Femoral Cartilage Repair: Episealer Twin Implantation Case Report. [PDF]

Case Rep Orthop
Ibrahim M, Wong I.
europepmc   +1 more source

The FOXC2‐LAMA4 Axis Orchestrates Vasculogenic Mimicry and Immunosuppressive Niche Formation to Drive Metastatic Cascade in Renal Cell Carcinoma

Advanced Science, EarlyView.
Advanced clear cell renal cell carcinoma metastasis is driven by a FOXC2+ tumor subpopulation. These cells execute vasculogenic mimicry for dissemination and secrete LAMA4. LAMA4 engages macrophage ITGA6, activating the STAT6/GATA3 axis to polarize macrophages into a pro‐metastatic, immunosuppressive phenotype.
Jiaxi Yao, Tong Xu, Chengyuan Wang, Chenyuan Wang, Junfeng Xie, Rui Zheng, Ke Wang, Xingzuo Jiang, Zewei Hu, Hongwei Jing, Lin Li, Tao Liu   +11 more
wiley   +1 more source

The Phylogenomic Framework and Infrageneric Classification of Temperate Asian Caraganeae (Leguminosae, Papilionoideae). [PDF]

Ecol Evol
Wang N, Liu PL, Zhang L, Ma R, Zhao L, Wang H, Chang ZY.   +6 more
europepmc   +1 more source

A Standard Operating Procedure for Protein Extraction From Abdominal Aortic Aneurysm Tissue: Enhancing Proteomics Applications. [PDF]

Proteomics Clin Appl
Baltazar T, Trindade F, Nogueira-Ferreira R, Vitorino R, Ferreira R, Domingues P, Leite-Moreira A, Dias-Neto M, VASCUL‐AID Consortium.   +8 more
europepmc   +1 more source

A nomogram to predict hypoxemia in the post-anesthesia care unit after laparoscopic bariatric surgery. [PDF]

BMC Anesthesiol
Wang M, Li Y, Gong Q, Xu Y, Chen X, Fan J.   +5 more
europepmc   +1 more source

Comparison of nanopore methods for respiratory clinical metagenomics

Doyle R, Vorslava V, Chang R, Leka B, Ward D, Batra R, Alcolea-Medina A, Snell LB.   +7 more
europepmc   +1 more source