Results 231 to 240 of about 91,527 (300)

Quantitative analysis of DNA‐GATA1 binding alterations linked to hematopoietic disorders

The FEBS Journal, EarlyView.
Native holdup allows the quantitative determination of affinities between full‐length transcription factors and DNA. Mutations in either the protein or the DNA can modulate binding strength, which can be precisely quantified using this approach. Applied to GATA1, it revealed mutations that alter DNA binding.
Boglarka Zambo, Bastien Morlet, Luc Negroni, Orsolya Mózner, Balázs Sarkadi, Gilles Travé, Gergo Gogl   +6 more
wiley   +1 more source

Comparison of Liquid Chromatography- and Nano-Electrospray Ionization-Mass Spectrometry Approaches for Single-Cell Metabolomics. [PDF]

Anal Chem
Cook A, Davison C, Pascoe J, Atwal H, Mayson G, Ali A, Beste DJ, Bailey M.   +7 more
europepmc   +1 more source

Degradomics for large‐scale mechanistic insights on proteases and proteolysis in human health

The FEBS Journal, EarlyView.
Proteolysis has an important role in human disease but remains relatively unexplored. Degradomics, the uncovering of proteolysis in tissues, cells, and proteins, uses mass spectrometry‐based terminomics to identify protein termini occurring therein (forward degradomics) and to define the actions of proteases (reverse degradomics).
Daniel R. Martin, Sumit Bhutada, Suneel S. Apte   +2 more
wiley   +1 more source

Spatially resolved mapping of histones reveals selective neuronal response in Rett syndrome

The FEBS Journal, EarlyView.
Loss of Mecp2 function is associated with Rett syndrome (RTT). MeCP2 regulates chromatin, yet its influence on histone composition and dynamics is unclear. Combining MALDI‐MSI with LCM–LC–MS/MS, we mapped histone proteoforms across the dentate gyrus, cornu ammonis, and cerebellum in two mouse models of RTT.
Frederike Schäfer, Guiseppa de Rocco, Moritz Völker‐Albert, Ignasi Forne, Nicoletta Landsberger, Axel Imhof, Shibojyoti Lahiri   +6 more
wiley   +1 more source

An Electrospray Sequential Mass Spectrometry Fragmentation Scheme of Erythromycin A and Its Application for the Elucidation of the Structures of Its Natural Co-Metabolites. [PDF]

Molecules
Jiang C, Gates PJ.
europepmc   +1 more source

OsCPN10a, cooperating with OsCPN20 and OsHSP60‐3B negatively regulate ABA signaling and enhance seed storability in rice

Journal of Integrative Plant Biology, EarlyView.
Abscisic acid signaling homeostasis is essential for seed storability. The molecular chaperone OsCPN10a enhances rice seed storability by forming a trimeric chaperone complex with OsCPN20‐OsHSP60‐3B that attenuates abscisic acid signaling via direct interaction with OsPYL10‐OsABIL1, thereby maintaining starch integrity and offering a promising target ...
Sufeng Liao, Yidong Wei, Yongsheng Zhu, Kunyang Li, Ting Chen, Shuai Zhao, Fangxi Wu, Jinlan Wang, Pengbo Yu, Hongguang Xie, Liping Chen, Qiuhua Cai, Huaan Xie, Jianfu Zhang   +13 more
wiley   +1 more source

Stable Carbon Isotope Analysis of Perfluorooctanoic Acid (PFOA) by Microflow-High Pressure Liquid Chromatography-Orbitrap Mass Spectrometry. [PDF]

Rapid Commun Mass Spectrom
Wojtal PK, Davidheiser-Kroll B, Lane CS, Mead RN.   +3 more
europepmc   +1 more source

Approach for quantitative analysis of hordein peptides in gluten-free malt beers with High-Resolution-Orbitrap-MS [PDF]

, 2017
Decloedt, Anneleen, Van Landschoot, Anita, Vanderputten, Dana, Watson, Hellen   +3 more
core   +1 more source

A posttranslational proteomic survey of a single anatomically preserved human 20‐week postconception brain

Journal of Anatomy, EarlyView.
A well‐preserved 20 post‐conception week human brain was obtained and finely dissected into 18 anatomically distinct regions, including the pia mater. Each region underwent in‐depth proteomic analysis, encompassing both total protein content and post‐translational modifications.
S. Bandiera, H. Bogetofte, P. Jensen, R. Hussain, S. A. Nischal, L. Rihova, G. J. Clowry, M. R. Larsen, Z. Molnár, B. C. Carlyle   +9 more
wiley   +1 more source

Pharmacokinetics of Intramuscular and Intravenous Glycopyrrolate in Juvenile New Zealand White Rabbits

Journal of Veterinary Pharmacology and Therapeutics, EarlyView.
ABSTRACT Glycopyrrolate is an alternative to atropine to treat bradyarrhythmias in anesthetized rabbits; however, there are no pharmacokinetic studies in the literature. Six female New Zealand White rabbits received glycopyrrolate 0.05 mg/kg intravenously (IV) in the cephalic vein or intramuscularly (IM) in a complete crossover design.
Jocelyn Marchiori, Andrea Sanchez, Kristen Messenger, Jennifer M. Reinhart, Hugues Beaufrère, Sonja Fonfara, Alexander Valverde, Yu Gu, Ron Johnson   +8 more
wiley   +1 more source