Results 311 to 320 of about 199,900 (336)
Some of the next articles are maybe not open access.
Titration of Lentivirus Vectors
Cold Spring Harbor Protocols, 2018The titer of a lentivirus vector is often expressed in transducing units per milliliter. This is a functional titer that reflects the lentivirus’ ability to transduce a particular cell line under specific conditions. Transduction of other cell lines is likely to be different and will require optimization.
Miguel, Sena-Esteves, Guangping, Gao
openaire +2 more sources
Lentivirus Replication and Regulation
Annual Review of Genetics, 1999▪ Abstract Lentiviruses are associated with chronic diseases of the hematological and neurological systems in animals and man. In particular, human immunodeficiency virus type 1 (HIV-1) is the etiological agent of the global AIDS epidemic. The genomes of lentiviruses are complex, encoding a number of regulatory and accessory proteins not found in ...
H, Tang, K L, Kuhen, F, Wong-Staal
openaire +2 more sources
2010
Lentiviral transgenesis is a promising alternative to direct microinjection of DNA into pronuclei, which is by and large restricted to certain mouse strains. Lentiviruses are complex retroviruses that integrate their genome into the host chromosome.
Alexander, Pfeifer +2 more
openaire +2 more sources
Lentiviral transgenesis is a promising alternative to direct microinjection of DNA into pronuclei, which is by and large restricted to certain mouse strains. Lentiviruses are complex retroviruses that integrate their genome into the host chromosome.
Alexander, Pfeifer +2 more
openaire +2 more sources
Journal of Virology, 1991
Transcriptional regulatory mechanisms found in lentiviruses employ RNA enhancer elements called trans-activation responsive (TAR) elements. These nascent RNA stem-loops are cis-acting targets of virally encoded Tat effectors. Interactions between Tat and TAR increase the processivity of transcription complexes and lead to efficient copying of viral ...
D, Derse +3 more
openaire +2 more sources
Transcriptional regulatory mechanisms found in lentiviruses employ RNA enhancer elements called trans-activation responsive (TAR) elements. These nascent RNA stem-loops are cis-acting targets of virally encoded Tat effectors. Interactions between Tat and TAR increase the processivity of transcription complexes and lead to efficient copying of viral ...
D, Derse +3 more
openaire +2 more sources
Lentivirus Production and Purification
2016Lentiviral (LV) vectors offer unique advantages over other gene delivery systems, namely the ability to integrate transgenes into the genome of both dividing and nondividing cells. Detailed herein is a simple protocol for the production LV vectors, describing the triple transfection of an LV transfer vector and LV helper plasmids into HEK-293 cells ...
Matthew J, Benskey +1 more
openaire +2 more sources
Gene delivery by lentivirus vectors
Molecular Biotechnology, 2007The capacity to efficiently transduce nondividing cells, shuttle large genetic payloads, and maintain stable long-term transgene expression are attributes that have brought lentiviral vectors to the forefront of gene delivery vehicles for research and therapeutic applications in a clinical setting.
Adam S, Cockrell, Tal, Kafri
openaire +2 more sources
Pathogenesis of lentivirus infections
Nature, 1986Following infection of animals or humans, lentiviruses play a prolonged game of hide and seek with the host's immune system which results in a slowly developing multi-system disease. Emerging knowledge of the disease processes is of some relevance to acquired immune deficiency syndrome (AIDS), which is caused by a virus possessing many of the ...
openaire +2 more sources
Lentivirus-Mediated Gene Expression
2007Lentiviruses have the capacity to enter and integrate their genetic material into cells that are not dividing. This property is retained in vectors based on these agents. They can thus effect gene delivery to cells that are difficult to transduce such as cardiac myocytes in vitro and in vivo.
Jing, Zhao, Andrew M L, Lever
openaire +2 more sources
Lentivirus-Mediated Antagomir Expression
2010MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation via hybridisation to mRNAs. miRNA function can be inhibited by the so-called "antagomirs" - anti-sense RNA oligonucleotides complementary to individual miRNAs.
Ewa, Surdziel +2 more
openaire +2 more sources
SIVsmmPBj14: An Atypical Lentivirus
1994SIV-PBj14 is atypical for a lentivirus in that infection of pig-tailed macaques usually does not result in long-term progressive disease; however, this model may potentially provide valuable information about the pathogenesis of HIV and the development of AIDS. By capitalizing on some of the unique properties of the virus and the model system discussed
openaire +2 more sources