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Recombination in yeast and the recombinant DNA technology
Genome, 1989The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
T D, Petes +9 more
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Introduction to recombinant DNA
Journal of Inherited Metabolic Disease, 1986AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
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DNA Recombineering Applications
Cold Spring Harbor Protocols, 2023The ability to manipulate the bacterial genome is an obligatory premise for the study of gene function and regulation in bacterial cells. The λ red recombineering technique allows modification of chromosomal sequences with base-pair precision without the need of intermediate molecular cloning steps.
Nara Figueroa-Bossi +2 more
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The recombinant DNA technology
Journal of Veterinary Pharmacology and Therapeutics, 1983The recombinant DNA technology or DNA cloning permits the isolation, amplification, and precise manipulation of specific DNA fragments. This is generally accomplished by linking or recombining the desired DNA fragment with a DNA molecule, termed the vector, which is capable of directing the replication of itself in a suitable host cell and any DNA ...
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Introduction to Recombinant DNA
Pediatrics, 1984Seldom has a scientific or biomedical break-through evoked the awe, controversy, or sheer incredulity that has accompanied the developments in the field of recombinant DNA technology or more popularly, gene cloning and genetic engineering. Now little more than one generation after Avery, et al1 demonstrated that genes were encoded in DNA and Watson and
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2006
The first step of the Adleman-Lipton extract model in DNA computing is the combinatorial generation of libraries. In this paper a new method is proposed for generating a initial pool, it is a quaternary recombination of strings via application of null context splicing rules.
FRANCO, Giuditta +3 more
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The first step of the Adleman-Lipton extract model in DNA computing is the combinatorial generation of libraries. In this paper a new method is proposed for generating a initial pool, it is a quaternary recombination of strings via application of null context splicing rules.
FRANCO, Giuditta +3 more
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Recombinant DNA and the pediatrician
The Journal of Pediatrics, 1981It is our opinion that the basic science clearly explained and illustrated in this article is surely worth understanding, in view of the several advances already established, the prospects which are imminent, and probably many other evolutions not yet anticipated.
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Recombination of DNA Molecules
1966Publisher Summary The chapter discusses the recombination of deoxyribonucleic acid (DNA) molecules. The chapter also discusses the major features of genetic recombination and also summarizes some current speculations on the recombination of DNA molecules.
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