Results 21 to 30 of about 894,247 (302)

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]

, 2012
Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA, Mehmet, H, Tchetchelnitski, V, Tolmachov, OE, Zambetti, LP   +4 more
core   +1 more source

An easy method to generate recombinant pseudorabies virus expressing the capsid protein of Porcine circovirus type 2d

Frontiers in Microbiology, 2023
IntroductionHomologous recombination is an effective way to generate recombinant viruses for vaccine research such as pseudorabies virus (PRV) and adenovirus.
Jingqiang Ren, Jingqiang Ren, Jingqiang Ren, Rachel Madera, Chase Cunningham, Jishu Shi, Lihua Wang   +6 more
doaj   +1 more source

A binary vector for transferring genomic libraries to plants [PDF]

, 1986
The transformation of mutant plants with a complete recombinant library derived from wild-type DNA followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes.
Simoens, Chris, Alliotte, Thierry, Mendel, R, Müller, A, Schiemann, J, Van Lijsebettens, Maria, Schell, Jeff, Van Montagu, Marc, Inzé, Dirk   +8 more
core   +1 more source

Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells [PDF]

, 2013
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells.
Apostolaki, Baldi, Beinke, Beinke, Beinke, Beinke, Beinke, Belich, Bouwmeester, Ciechanover, Cohen, Coope, Costa, Dauber, Dumitru, Durocher, Elke MÜHLBERGER, Feng, Gantke, Geisse, George, Gray, Jia, Jia, Julia Janzen, Kabouridis, Kaufman, Lang, Liu, Nicholas A. Morrice, Papoutsopoulou, Pomerantz, Robinson, Roget, Sabrina Boussouf, Salmeron, Salmeron, Savinova, Schlaeger, Schumann, Steven Howell, Steven C. Ley, Thorsten Gantke, Waterfield, Waterfield, Wu, Yang, Zhao   +47 more
core   +1 more source

Gel-Based Purification and Biochemical Study of Laccase Isozymes from Ganoderma sp. and Its Role in Enhanced Cotton Callogenesis

Frontiers in Microbiology, 2017
Basidiomycetous fungi, Ganoderma lucidum MDU-7 and Ganoderma sp. kk-02 secreted multiple laccase isozymes under diverse growth condition. Aromatic compounds and metal salts were also found to regulate the differential expression of laccase isozymes from ...
Krishna K. Sharma, Amit Kumar, Amit Kumar, Deepti Singh, Sakshi Arora, Amarjeet K. Singh, Sarvajeet S. Gill, Barkha Singhal   +7 more
doaj   +1 more source

Mucosal Application of gp140 Encoding DNA Polyplexes to Different Tissues Results in Altered Immunological Outcomes in Mice [PDF]

, 2013
Increasing evidence suggests that mucosally targeted vaccines will enhance local humoral and cellular responses whilst still eliciting systemic immunity.
A Azizi, A Kantele, A Minne, BA Koblin, C Lindholm, CJ Wei, CM Rush, DL Woodland, H Jiang, J De Calisto, J Holmgren, Jamie F. S. Mann, JF Mann, JF Richmond, JH Song, John S. Tregoning, John Stambas, JR Mora, L Buonaguro, L Torrieri-Dramard, L Wu, LS Klavinskis, M Bivas-Benita, MA Arias, MR Neutra, Paul F. McKay, R Geiben-Lynn, Reeyeshkumar K. Patel, Robin J. Shattock, S Lu, S Mazumder, S Wang, Samantha Arokiasamy, SW Barnett, XD Yao, Z Wang   +35 more
core   +13 more sources

MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA [PDF]

, 2012
Topoisomerase II creates a double-strand break intermediate with topoisomerase covalently coupled to the DNA via a 5'-phosphotyrosyl bond. These intermediate complexes can become cytotoxic protein-DNA adducts and DSB repair at these lesions requires ...
Austin, Caroline, Cockell, Simon, Cowell, Ian, Curtis, Hannah, Jackson, Graham, Lee, Ka Cheong, Moiani, Davide, Morris, Nicholas, Padget, Kay, Sondka, Zbyslaw, Tainer, John   +10 more
core   +3 more sources

Label-free electrochemical monitoring of DNA ligase activity [PDF]

, 2008
This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I., Bellamy S. R., Borodina T. A., Bullard D. R., Cahova-Kucharikova K., Cao W. G., Doherty A. J., Fadrna R., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Friedberg E. C., Gowers D. M., Heiter D. F., Kucharikova K., Liu L. F., Palecek E., Pascal J. M., Rass U., Schrock R. D., Scott B. O. S., Shuman S., Tomkinson A. E., Vacek J., Wilkinson A., Wilkinson A., Zauner G.   +29 more
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Staphylococcus aureus Protection-Related Type 3 Cell-Mediated Immune Response Elicited by Recombinant Proteins and GM-CSF DNA Vaccine

Vaccines, 2021
Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α
Kamila R. Santos, Fernando N. Souza, Eduardo M. Ramos-Sanchez, Camila F. Batista, Luiza C. Reis, Wesley F. Fotoran, Marcos B. Heinemann, Hiro Goto, Magnus Gidlund, Adriano F. Cunha, Angélica Rosa Faria, Hélida M. Andrade, Andrey P. Lage, Mônica M. O. P. Cerqueira, Alice M. M. P. Della Libera   +14 more
doaj   +1 more source

PARP-3 and APLF function together to accelerate nonhomologous end joining [PDF]

, 2010
PARP-3 is a member of the ADP-ribosyl transferase superfamily of unknown function. We show that PARP-3 is stimulated by DNA double-strand breaks (DSBs) in vitro and functions in the same pathway as the poly (ADP-ribose)-binding protein APLF to accelerate
Ahel, Ame, Anna E.O. Fisher, Audebert, Bekker-Jensen, Benjamin, Bernardo Reina-San-Martin, Boulton, Brown, Bryant, Bryant, Caldecott, Caldecott, Critchlow, Ding, Drouet, El-Khamisy, Farmer, Fisher, Grawunder, Guy Poirier, Hochegger, Hottiger, Iles, Isabelle Robert, Kanno, Keith W. Caldecott, Kickhoefer, Kleine, Le Page, Lehtio, Limei Ju, Lobrich, Loseva, Macrae, Mahaney, Maria C. Zuma, Mehrotra, Michele Rouleau, Morrison, Okano, Otto, Poirier, Robert, Roberts, Rogakou, Rogakou, Rouleau, Rulten, Smith, Soulas-Sprauel, Stevnsner, Stuart L. Rulten, Sugimura, Trucco, Wang, Yan, Yang, Yano, Yano   +59 more
core   +1 more source