Results 51 to 60 of about 4,366,484 (267)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

The effect of calcium on the growth of native species in a tropical forest hotspot

open access: yesiForest - Biogeosciences and Forestry, 2018
Recovering of degraded areas depends not only on the choice of native species to be planted, but also on the requirements of planted seedling species in terms of soil fertility, mainly in tropical areas.
Gonçalves Bizuti DT   +5 more
doaj   +1 more source

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

open access: yesFEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi   +6 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Model United Nations: Improving the Students' Speaking Skill

open access: yesJEES (Journal of English Educators Society), 2019
Model United Nations promotes students’ learning opportunities in three successive ways: optimizing individual development through un-moderated caucus, growing competitiveness through working paper forum and sharpening problem solving skill through draft
Sukma Septian Nasution   +1 more
doaj   +1 more source

Improving the Caesarean Decision by Robson Classification: A Population-Based Study by 5,323,500 Livebirth Data

open access: yesAnnals of Global Health, 2020
Background: Caesarean section is a major obstetric intervention for saving lives of women and their newborns from pregnancy- and childbirth-related complications. C-Section rate is considered an important indicator for measuring obstetric services in any
H. Omer Tontus, Saniye Nebioglu
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Two Cuneiform Texts Found in Acemhöyük from the Old Assyrian Period

open access: yesBelleten, 2016
Acemhöyük, which is located within the boundaries of Yeşilova Town, approximately 18 km to the northwest of Aksaray, one of the most important archaeological centers of Anatolia.
Remzi Kuzuoğlu
doaj   +1 more source

COVID Nursing and PTSD

open access: yesPatient Safety, 2021
Content warnings: mental health and suicidal ideation I didn’t want to be able to write this essay. In fact, I would hazard that no one wants to be able to write about how their job gave them a debilitating mental illness that endangered their life and ...
Cassandra Alexander
doaj   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

open access: yesFEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim   +11 more
wiley   +1 more source

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