Results 41 to 50 of about 8,618 (173)

An improved spectrophotometric assay for leucine aminopeptidase [PDF]

open access: yesBiochemical Journal, 1987
A sensitive assay to determine the activity of leucine aminopeptidase (EC 3.4.11.1), using L-leucine thiobenzyl ester as substrate, was developed. Hydrolysis of the ester by leucine aminopeptidase can be monitored in the presence of 5,5-dithiobis-(2-nitrobenzoic acid) by continuous spectrophotometric measurement at 412 nm.
R E, Beattie   +4 more
openaire   +2 more sources

Root Order Dependent Changes in Fine‐Root Substrate and Soil Environment Regulate Soil Organic Nitrogen Fractions Under Acid Rain and Nitrogen Deposition

open access: yesLand Degradation &Development, EarlyView.
ABSTRACT Acid rain and nitrogen deposition are important environmental stressors affecting soil nitrogen cycling in subtropical forests, whereas fine‐root decomposition represents a major pathway for soil organic nitrogen (SON) input and transformation.
Xiongfei Zhang   +10 more
wiley   +1 more source

Effects of polystyrene nanoparticles on the microbiota and functional diversity of enzymes in soil

open access: yesEnvironmental Sciences Europe, 2018
Background The increasing production of nanoplastics and the fragmentation of microplastics into smaller particles suggest a plausible yet unclear hazard in the natural environment, such as soil.
T. T. Awet   +9 more
doaj   +1 more source

Carbon‐Fixing Bacterial Network Complexity Mediates Soil Multifunctionality in a Panax notoginseng‐Pinus armandii Agroforestry System

open access: yesLand Degradation &Development, EarlyView.
ABSTRACT Autotrophic carbon‐fixing bacteria (CFB) are central contributors to soil carbon sequestration and biogeochemical carbon cycling in agroforestry ecosystems and can act as reliable biological indicators for evaluating agroforestry ecosystem sustainability.
Jingying Hei   +3 more
wiley   +1 more source

Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase

open access: yesAdsorption Science & Technology, 2021
Soil leucine aminopeptidase (LAP) is a hydrolytic enzyme involved in the acquisition of nitrogen by microorganisms. In contaminated soils, LAP activity is affected not only by the type and concentration of heavy metals but also by the form of enzyme ...
Shunyu Huang, Jingji Li, Jipeng Wang
doaj   +1 more source

Plasmepsins as Antimalarial Drug Targets—Then, Now, and the Future

open access: yesMedicinal Research Reviews, EarlyView.
ABSTRACT Malaria is a devastating disease caused by Plasmodium parasites. Plasmodium parasites express ten cathepsin D‐like aspartyl proteases, called plasmepsins (PMs). These PMs have diverse roles fulfill diverse functions throughout the parasite's lifecycle, though several exhibit functional redundancies. Among them, PMV, PMIV, and PMX are essential
Brad E. Sleebs
wiley   +1 more source

Degradable versus inert microplastics: Effects on soil organic carbon persistence via microbial drivers in an agroecosystem

open access: yesFunctional Ecology, EarlyView.
Read the free Plain Language Summary for this article on the Journal blog. Abstract Microplastics (MPs) are widespread in terrestrial ecosystems, raising global concerns that they may disrupt soil organic carbon (SOC) cycling and undermine agroecosystem contributions to climate mitigation.
Meng‐Ying Li   +11 more
wiley   +1 more source

Changes in the Levels of Neuropeptides and Their Metabolizing Enzymes in the Brain Regions of Nucleus Basalis Magnocellularis-Lesioned Rats

open access: yesJournal of Pharmacological Sciences, 2003
The regulation mechanism of the interrelation between neuropeptides and their metabolizing enzymes in in vivo tissues is still not clear. In the present report, we attempted to measure the levels of neuropeptides and their enzymes in the frontal cortex ...
Masaru Yamamoto   +2 more
doaj   +1 more source

The tomato chloroplast stromal proteome compendium elucidated by leveraging a plastid protein-localization prediction Atlas

open access: yesFrontiers in Plant Science, 2023
Tomato (Solanum lycopersicum) is a model species for studying fruit development, wounding, herbivory, and pathogen attack. Despite tomato’s world-wide economic importance and the role of chloroplasts as metabolic hubs and integrators of environmental ...
Oindrila Bhattacharya   +4 more
doaj   +1 more source

Bio‐organic fertilizer alleviated soil microbial C limitations, aggravated P limitations and increased microbial C use efficiency in a degraded desert steppe

open access: yesFunctional Ecology, EarlyView.
Read the free Plain Language Summary for this article on the Journal blog. Abstract Bio‐organic fertilizer (BOF), which integrates organic carbon sources, beneficial microorganisms and essential nutrients, represents a distinctive approach to grassland restoration that differs fundamentally from conventional fertilization practices.
Yao Zhou   +7 more
wiley   +1 more source

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