Results 71 to 80 of about 3,639,878 (311)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Open Access – What’s New in the Sciences?

open access: yes, 2015
Learn about some of the newest open access publications and platforms designed to support and accelerate science research. Join a discussion with librarian liaisons to science departments about how Gettysburg faculty and the library are ...
Scholarly Communications,
core  

Experiences with the Greenstone digital library software for international development [PDF]

open access: yes, 2010
Greenstone is a versatile open source multilingual digital library environment, emerging from research on text compression within the New Zealand Digital Library Research Project in the Department of Computer Science at the University of Waikato. In 1997
Bainbridge, David   +3 more
core   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

open access: yesFEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu   +10 more
wiley   +1 more source

nanopub-java: A Java Library for Nanopublications [PDF]

open access: yes, 2015
The concept of nanopublications was first proposed about six years ago, but it lacked openly available implementations. The library presented here is the first one that has become an official implementation of the nanopublication community.
Kuhn, Tobias
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

UCL (University College London) Libraries Masterplan: Library Report to Estates Management Committee January 2008 [PDF]

open access: yes, 2008
This document is a Report from UCL Library Services to UCL on Master Planning activities and outputs which have been undertaken to quantify use and development of estate in UCL Library Services.
Ayris, P.   +1 more
core  

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

open access: yesFEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho   +4 more
wiley   +1 more source

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