Results 191 to 200 of about 6,867,278 (300)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Autism Spectrum Disorder and Life Expectancy Among Medicaid Beneficiaries.

JAMA Netw Open
Li G, DiGuiseppi CG, Blanchard A, Russell MT, Ing C.   +4 more
europepmc   +1 more source

Associations of Multiomics Biological Aging With Diabetic Retinopathy and Life Expectancy. [PDF]

Invest Ophthalmol Vis Sci
Yu J, Gao YL, Zhang Y, Xu JM, Chen H, Ho M, Kam KW, Young AL, Pang CP, Tham CC, Yam JC, Chen LJ.   +11 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Medical Maximizing Tendencies and Acceptance of Life Expectancy-Based Cancer Screening.

JAMA Netw Open
Brotzman LE, Kullgren JT, Malani PN, Solway ES, Kirch MA, Singer DC, Roberts JS, Zikmund-Fisher BJ.   +7 more
europepmc   +1 more source

Life expectancy in open spina bifida. [PDF]

Dev Med Child Neurol
Shavelle RM, Paige MK, Brooks JC, Strauss DJ.   +3 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Weekend Warrior and other physical activity patterns: mortality and life expectancy in individuals with cardiometabolic multimorbidity. [PDF]

J Exerc Sci Fit
Huang Y, Jiang Y, Liu S, Chen C, You Y, Wang Y, Liao S, Wang Q, Chen L.   +8 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

The moderating effect of age on loneliness and subjective remaining life expectancy among Chinese adults. [PDF]

Front Public Health
Che SL, Leong SM, Tam KI.
europepmc   +1 more source