Results 91 to 100 of about 10,801,097 (341)

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Natural History, Quality of Life, and Outcome in Cardiac Transthyretin Amyloidosis.

Circulation, 2019
BACKGROUND Transthyretin amyloidosis cardiomyopathy (ATTR-CM) is an increasingly recognized cause of heart failure in older individuals. We sought to characterize the natural history of ATTR-CM and compare outcomes and quality of life among patients with
T. Lane, M. Fontana, A. Martinez-Naharro, C. Quarta, C. Whelan, A. Petrie, D. Rowczenio, J. Gilbertson, D. Hutt, T. Rezk, Svetla G Strehina, Joan Caringal-Galima, R. Manwani, F. Sharpley, A. Wechalekar, H. Lachmann, S. Mahmood, S. Sachchithanantham, E. Drage, Harvey Jenner, R. McDonald, Ottavia Bertolli, A. Calleja, P. Hawkins, J. Gillmore   +24 more
semanticscholar   +1 more source

P‐glycoprotein modulates the fluidity gradient of the plasma membrane of multidrug resistant CHO cells

FEBS Letters, EarlyView.
To explore the impact of the overexpression of the multidrug‐transporter P‐glycoprotein (ABCB1) on membrane fluidity, we compared the transversal gradient of mobility and microviscosity in plasma membranes of drug‐sensitive Chinese hamster ovary cells (AuxB1) and their multidrug‐resistant derivatives (B30) using the fluorescent n‐(9‐anthroyloxy) fatty ...
Roger Busche, John R. Riordan, Burkhard Tümmler   +2 more
wiley   +1 more source

Local biodiversity is higher inside than outside terrestrial protected areas worldwide

Nature Communications, 2016
Protected areas are thought essential for biodiversity conservation, but few studies confirm that protection benefits species. Here, Gray and Hill et al. analyse a global, taxonomically broad database to show that local species richness and abundance are
Claudia L. Gray, Samantha L. L. Hill, Tim Newbold, Lawrence N. Hudson, Luca Börger, Sara Contu, Andrew J. Hoskins, Simon Ferrier, Andy Purvis, Jörn P. W. Scharlemann   +9 more
doaj   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection

Parasites & Vectors, 2015
Background Accurate diagnosis of urogenital schistosomiasis is vital for surveillance/control programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but requires infrastructure, financial resources and skilled personnel ...
A. Rosser, D. Rollinson, M. Forrest, B L Webster   +3 more
doaj   +1 more source

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

FEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt, Marcus S. Münchhoff, Jacqueline Krohn, Philip M. Palarz, Manuel H. Taft, Johannes N. Greve, Nataliya Di Donato, Falk F. R. Buettner, Dietmar J. Manstein   +8 more
wiley   +1 more source

Anchonini in Africa: New Species and Genus Confirming a Transatlantic Distribution (Coleoptera: Curculionidae: Molytinae)

Diversity, 2018
The Anchonini known from Africa are reviewed. The monotypic genus Aethiopacorep is redescribed. The new West African genus Titilayo gen. nov. is described, with seven new species: four from São Tomé, T. perrinae sp. nov., T. saotomense sp.
Joana P. Cristóvão, Christopher H. C. Lyal   +1 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source