Results 21 to 30 of about 5,652 (193)
Structure-Guided Engineering of the Regioselectivity of RNA Ligase Ribozymes [PDF]
Journal of the American Chemical Society, 2009 Ribozyme-catalyzed RNA synthesis is central to the RNA world hypothesis. No natural RNA polymerase ribozymes have been discovered. However, ribozymes that catalyze the requisite chemistry, generating a new phosphodiester through attack of a terminal hydroxyl of an RNA on the alpha-phosphate of a triphosphate-activated oligonucleotide, have been ...
Jason N. Pitt, A.R. Ferré-D′Amaré
openalex +3 more sources
Direct selection of trans -acting ligase ribozymes by in vitro compartmentalization [PDF]
RNA, 2005 We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans -acting variant of the Bartel class I ligase which ...
Matthew Levy +2 more
openalex +3 more sources
Assembly of a Ribozyme Ligase from Short Oligomers by Nonenzymatic Ligation
Journal of the American Chemical Society, 2020 Our current understanding of the chemistry of the primordial genetic material is fragmentary at best. The chemical replication of oligonucleotides long enough to perform catalytic functions is particularly problematic because of the low efficiency of nonenzymatic template copying.
Lijun Zhou +2 more
openalex +3 more sources
A natural ribozyme with 3′,5′ RNA ligase activity [PDF]
Nature Chemical Biology, 2009 Using electrophoresis, sequencing and enzymatic digestion, we show that the group I intron from the cyanobacterium Anabaena sp. PCC 7120 catalyzes phosphodiester bond formation using a triphosphate on the 5'-terminal nucleotide, much like protein polymerases and engineered ribozymes.
Quentin Vicens, Thomas R. Cech
openalex +3 more sources
Design and optimization of effector-activated ribozyme ligases [PDF]
Nucleic Acids Research, 2000 A selected ribozyme ligase, L1, has been engineered to respond to small organic effectors. Residues important for ribozyme catalysis were mapped to a compact core structure. Aptamers that bound adenosine and theophylline were appended to the core structure, and the resultant aptazymes proved to be responsive to their cognate effectors.
Mark P. Robertson
openalex +3 more sources
A self-replicating ligase ribozyme [PDF]
Proceedings of the National Academy of Sciences, 2002 A self-replicating molecule directs the covalent assembly of component molecules to form a product that is of identical composition to the parent. When the newly formed product also is able to direct the assembly of product molecules, the self-replicating system can be termed autocatalytic.
Natasha Paul, Gerald F. Joyce
openalex +3 more sources
In vitro selection of ribozyme ligases that use prebiotically plausible 2-aminoimidazole–activated substrates [PDF]
Proceedings of the National Academy of Sciences, 2020 Significance Current models for the origin of life include an earlier period when prebiotic chemistry dictated the nonenzymatic copying of RNA polymers, followed by a period of ribozyme-catalyzed reactions using nucleoside-5′-triphosphate (NTP) substrates. Our study addresses the transition from nonenzymatic to ribozyme-catalyzed RNA template
Travis Walton +4 more
openalex +4 more sources
Emergent Chemical Reactivity and Complexity of RNA Condensates. [PDF]
Angew Chem Int Ed EnglRNA condensates capable of substrate enrichment and catalysis modulate their environment. Could this start a catalytic selection loop or evolution? Abstract The RNA world hypothesis posits the existence of life‐like assemblies that consisted mostly of RNA.
Hauf S +4 more
europepmc +2 more sources
Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst? [PDF]
ChembiochemRational design is a potent strategy for developing ribozymes with advanced functionalities. Engineered variants of the hairpin ribozyme impressively demonstrate how function can be tuned through structural manipulation. The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention ...
Ebermann C, Müller S.
europepmc +2 more sources
Evolutionary Dynamics in Molecular Populations of Ligase Ribozymes
, 2000 The emergence of life depended on the ability of the first biopolymer populations to thrive and approach larger population sizes and longer sequences that could store enough information, as required for a cellular type of life. The evolution of these populations very likely occurred under circumstances under which Muller’s Ratchet in synergism with ...
Carolina Díaz Arenas
openalex +3 more sources