Results 21 to 30 of about 5,438 (161)
Viroids: Survivors from the RNA World? [PDF]
, 2014 Because RNA can be a carrier of genetic information and a biocatalyst, there is a consensus that it emerged before DNA and proteins, which eventually assumed these roles and relegated RNA to intermediate functions.
Flores, Ricardo +3 more
core +1 more source
RNA-Catalyzed RNA Ligation on an External RNA Template [PDF]
, 2002 Variants of the hc ligase ribozyme, which catalyzes ligation of the 3′ end of an RNA substrate to the 5′ end of the ribozyme, were utilized to evolve a ribozyme that catalyzes ligation reactions on an external RNA template. The evolved ribozyme catalyzes
Joyce, Gerald F, McGinness, Kathleen E
core +1 more source
Force for ancient and recent life: viral and stem-loop RNA consortia promote life. [PDF]
, 2014 Lytic viruses were thought to kill the most numerous host (i.e., kill the winner). But persisting viruses/defectives can also protect against viruses, especially in a ubiquitous virosphere. In 1991, Yarmolinsky et al.
Villarreal, Luis P
core +1 more source
Beyond seek and destroy: How to generate allelic series using genome editing tools [PDF]
, 2020 Genome editing tools have greatly facilitated the functional analysis of genes of interest by targeted mutagenesis. Many usable genome editing tools, including different site-specific nucleases and editor databases that allow single-nucleotide ...
Bes, Martine +10 more
core +2 more sources
Frontiers in Microbiology, 2018 Eggplant latent viroid (ELVd) is a relatively small non-coding circular RNA that induces asymptomatic infections in eggplants (Solanum melongena L.). Like other viroid species that belong to the family Avsunviroidae, ELVd contains hammerhead ribozymes in
Teresa Cordero +2 more
doaj +1 more source
Isolation of novel ribozymes that ligate AMP-activated RNA substrates [PDF]
, 1997 Background: The protein enzymes RNA ligase and DNA ligase catalyze the ligation of nucleic acids via an adenosine-5′-5′-pyrophosphate ‘capped’ RNA or DNA intermediate. The activation of nucleic acid substrates by adenosine 5′-monophosphate (AMP) may be a
Hager, Alicia J., Szostak, Jack W.
core +1 more source
Large Phenotypic Enhancement of Structured Random RNA Pools. [PDF]
, 2020 Laboratory evolution of functional RNAs has applications in many areas of chemical and synthetic biology. In vitro selections critically depend on the presence of functional molecules, such as aptamers and ribozymes, in the starting sequence pools.
Chizzolini, Fabio +5 more
core
Molecules, 2010 In vitro selection with long random RNA libraries has been used as a powerful method to generate novel functional RNAs, although it often requires laborious structural analysis of isolated RNA molecules.
Yuki Fujita +3 more
doaj +1 more source
Rolling-circle replication of viroids, viroid-like satellite RNAs and hepatitis delta virus: variations on a theme [PDF]
, 1999 Viroids and viroid-like satellite RNAs from plants, and the human hepatitis delta virus (HDV) RNA share some properties that include small size, circularity and replication through a rolling-circle mechanism.
Flores, Ricardo +5 more
core +1 more source
Biology Direct, 2007 Background The origin of the translation system is, arguably, the central and the hardest problem in the study of the origin of life, and one of the hardest in all evolutionary biology.
Koonin Eugene V, Wolf Yuri I
doaj +1 more source