Cold Spring Harbor Protocols, 2019 DNA ligases are used chiefly to create novel combinations of nucleic acid molecules and to attach them to vectors before molecular cloning. They are either of bacterial origin or bacteriophage encoded and have different properties, as discussed here.
Michael R, Green, Joseph, Sambrook
openaire +2 more sources
Mini-loop ligation of a bleeding duodenal Dieulafoy's lesion [PDF]
, 2013 Two percent of gastrointestinal hemorrhages are caused by Dieulafoy's lesions, which are located in duodenum in only 15% of cases. There are no recommendations regarding the prime endoscopic treatment technique for this condition.
Ljubičić, Neven +1 more
core +1 more source
Comparison and transfer testing of multiplex ligation detection methods for GM plants [PDF]
, 2012 Background With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO ...
van Dijk Jeroen P +37 more
core +1 more source
Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning [PDF]
, 2010 Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker +15 more
core +1 more source
Robust detection and identification of multiple oomycetes and fungi in environmental samples by using a novel cleavable padlock probe-based ligation-detection assay [PDF]
, 2009 Simultaneous detection and identification of multiple pathogenic microorganisms in complex environmental samples are required in numerous diagnostic fields.
Dullemans, A.M. +10 more
core +2 more sources
A Serine Ligation Method Based on Mitsunobu Reaction and O-to-N Acyl Transfer [PDF]
, 2023 A ligation protocol has been developed in which a peptide containing an N-terminal serine is linked via its side-chain alcohol to the C-terminal carboxylate of a second peptide by Mitsunobu esterification.
Michael, Kopach +4 more
core +1 more source
Simultaneous microsurgical spermatic vein ligation and sclerotherapy - A combined procedure for the treatment of recurrent or persistent varicocele [PDF]
, 2001 Objectives: Microsurgical ligation as well as antegrade sclerotherapy have been established in varicocele treatment. The aim of this study was to evaluate whether a combination of microsurgery.
Corvin, Stefan +6 more
core +1 more source
Catalysis of Hydrazone and Oxime Peptide Ligation by Arginine [PDF]
, 2020 Hydrazone and oxime peptide ligations are catalyzed by arginine. The catalysis is assisted intramolecularly by the side-chain guanidinium group. Hydrazone ligation in the presence of arginine proceeds efficiently in phosphate buffer at neutral pH but is ...
Jérôme, Vicogne +6 more
core +2 more sources
A Rectovaginal Fistula Treated by Ligation of Intersphincteric Fistula Tract Method
Acta Medica Alanya, 2018 Rectovaginal fistulas are epithelial-lined communications between the rectum and vagina. A number of surgical options are available for patients with rectovaginal fistulas. In this manuscript, a case having rectovaginal fistula and treated by ligation of
Hasan Cali̇s, Nuraydin Ozlem
doaj +1 more source
Veterinary Medicine and Science, 2023 Objective The objective of this study was to evaluate differences in outcomes in dogs treated for extrahepatic portosystemic shunts (EHPSS) by either complete suture ligation, partial suture ligation or medical management.
Rebecca L. Beardall +3 more
doaj +1 more source