Results 61 to 70 of about 401,398 (215)

Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reactions

Molecules, 2011
A new simple scheme for constructing recombinant vectors that does not require any restriction enzyme, ligase, or any other special enzyme treatment has been developed.
Akinori Kuzuya, Keita Tanaka, Hitoshi Katada, Makoto Komiyama   +3 more
doaj   +1 more source

Induction of Myocardial Infarction in Experimental Animals: A Review [PDF]

Journal of Clinical and Diagnostic Research, 2018
Myocardial Infarction (MI) is a major health problem worldwide. Animal models have been used extensively in scientific research. This was intended to help researchers to understand the underlying pathophysiology of MI, compensatory mechanisms involved ...
Syarifah Aisyah Syed Abd Halim, Norzana Abd Ghafar, Zakiah Jubri, Srijit Das   +3 more
doaj   +1 more source

Ligation-Rolling Circle Amplification on Quantum Dot-Encoded Microbeads for Detection of Multiplex G‑Quadruplex-Forming Sequences

, 2018
The combination of microbead array with assay chemistry of isothermal amplification enables the continuous development of nucleic acid detection techniques.
Feika Bian (3217182), Xuebin Huang (2037007), Qinyu Ge (216523), Qingjiang Sun (1413766), Feika Bian, Qingjiang Sun, Qingsheng Guo, Xiaojun Qu, Qinyu Ge, Xiaojun Qu (1413760), Qingsheng Guo (668687), Xuebin Huang   +11 more
core   +1 more source

Azole reagents enabled ligation of peptide acyl pyrazoles for chemical protein synthesis

, 2023
Native chemical ligation (NCL) has been playing an increasingly important role in chemical protein synthesis (CPS), more efficient ligation methods that circumvent the requirement of peptidyl thioester and thiol additive—which allow the following ...
Chunmao, He, Peisi, Liao
core   +1 more source

Ligation-Dependent Cas14a1-Activated Biosensor for One-pot Pathogenic Diagnostic

, 2022
Pathogenic identification requires nucleic acid diagnosis with simple equipment and fast manipulation. Our work established an all-in-one strategy assay with excellent sensitivity and high specificity, Transcription-Amplified Cas14a1-Activated Signal ...
Xidan, Luo, Wenkai, Shang, Yi, Wan, Yuefeng, Qiao, Jinghong, Li, Xiufen, Yang, Huiying, Cai, Fengge, Song, Xiao, Tan, Hongwei, Hou, Yangdao, Wei   +10 more
core   +1 more source

Anterior Ethmoid Artery Ligation for Epistaxis: A Systematic Review

Laryngoscope Investigative Otolaryngology
Objective For intractable epistaxis unresponsive to conservative measures, surgical intervention, such as anterior ethmoid artery ligation (AEAL), can control bleeding in the superior nasal cavity. Yet, the literature is limited around AEAL for epistaxis
Wynne Zheng, Christina Zhu, Jiling Chou, Jason Crossley, Timothy R. DeKlotz, Daniel B. Spielman   +5 more
doaj   +1 more source

Directional high-throughput sequencing of RNAs without gene-specific primers

BioTechniques, 2018
Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing.
Anita Mäki, Marja Tiirola
doaj   +1 more source

Orbital Varix and Its Surgical Correction - A Case Report

Indian Journal of Plastic Surgery, 1993
A Young patient presented with left unilateral proptosis and Orbital venography was suggestive of orbital vari on left side. An easy and effective operative procedure was planned. The superficial veins around orbit were ligated. Result was acceptable and
Dr. P.K. Sharma, Dr. B. Shukla, Dr. B.M. Tripathi, Dr. R. Malik   +3 more
doaj   +1 more source

Chemical Ligation for Molecular Imaging

Molecular imaging in living systems has become an important tool for understanding molecular processes and cellular events in preclinical settings.
Karatas, Hacer, Budin, Ghyslain, Dubikovskaya, Elena A., Godinat, Aurélien   +3 more
core   +1 more source

Determinação do GL50 em dois modelos de sepse experimental [PDF]

, 2012
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Ciências Médicas, Florianópolis, 2012INTRODUÇÃO Ainda não foi descrito um modelo experimental ideal de para estudo da sepse.
Locks, Giovani de Figueiredo
core