Results 41 to 50 of about 870,748 (257)

The large extracellular loop of CD63 interacts with gp41 of HIV-1 and is essential for establishing the virological synapse

Scientific Reports, 2021
Human immunodeficiency virus type 1 (HIV-1) persists lifelong in infected individuals and has evolved unique strategies in order to evade the immune system. One of these strategies is the direct cell-to-cell spread of HIV-1.
Daniel Ivanusic, Kazimierz Madela, Norbert Bannert, Joachim Denner   +3 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Serial block-face scanning electron microscopy of adherent cells on thin plastic substrate

Methods in Microscopy
Serial block-face (SBF) scanning electron microscopy (SEM) is used for imaging the entire internal ultrastructure of cells, tissue samples or small organisms.
Kauter Anne, Bürge Silvio, Klotz Christian, Laue Michael   +3 more
doaj   +1 more source

A solid‐phase transfection platform for arrayed CRISPR screens

Molecular Systems Biology, 2019
Arrayed CRISPR‐based screens emerge as a powerful alternative to pooled screens making it possible to investigate a wide range of cellular phenotypes that are typically not amenable to pooled screens. Here, we describe a solid‐phase transfection platform
Özdemirhan Serçin, Sabine Reither, Paris Roidos, Nadja Ballin, Spyridon Palikyras, Anna Baginska, Katrin Rein, Maria Llamazares, Aliaksandr Halavatyi, Hauke Winter, Thomas Muley, Renata Z Jurkowska, Amir Abdollahi, Frank T Zenke, Beate Neumann, Balca R Mardin   +15 more
doaj   +1 more source

Uncovering Differently Expressed Markers and Heterogeneity on Human Pancreatic Cancer

Translational Oncology, 2020
Discovery of biomarkers is critical to understand tumor heterogeneity and microenvironment. To determine differently expressed makers on cancer tissue for comprehensive profiling, the multiplexed tissue imaging mass cytometer (IMC) which uniquely ...
Sorah Yoon, Haiqing Li, Loren Quintanar, Brian Armstrong, John J Rossi   +4 more
doaj   +1 more source

Rare isolation of human-tropic recombinant porcine endogenous retroviruses PERV-A/C from Göttingen minipigs

Virology Journal, 2022
Background Porcine endogenous retroviruses (PERVs) can infect human cells and pose a risk for xenotransplantation when pig cells, tissues or organs are transplanted to human recipients.
Sabrina Halecker, Ludwig Krabben, Yannick Kristiansen, Luise Krüger, Lars Möller, Dietmar Becher, Michael Laue, Benedikt Kaufer, Christian Reimer, Joachim Denner   +9 more
doaj   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Manufacturing and processing of sheets using a Mg–Al–Ca–Zn–Y alloy for automotive applications

Results in Engineering
As the transportation industry evolves, so do its needs, creating opportunities for the use of non-standard materials. While electrically powered vehicles (EVs) generally operate at lower temperatures than those with internal combustion engines ...
Stefan Gneiger, Nikolaus Papenberg, Stefan Mitsche, Martin Fehlbier   +3 more
doaj   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Recruitment of the cellular lipid transport protein CERT to C. psittaci inclusions regulates the timing of bacterial egress

Scientific Reports
Egress of intracellular pathogens is highly regulated and carefully timed. For the zoonotic bacterium C. psittaci, the predominant egress pathway is Chlamydia-containing sphere (CCS) formation, a calcium-dependent sequential mechanism including protease ...
Jana Scholz, Gudrun Holland, Michael Laue, Sebastian Banhart, Dagmar Heuer   +4 more
doaj   +1 more source