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Light sheet fluorescence microscopy with active optical manipulation.
Optics LettersWe present a light sheet fluorescence microscopy (LSFM) with active optical manipulation by using linear optical tweezers (LOTs). In this method, two coaxially transmitting laser beams of different wavelengths are shaped using cylindrical lenses to form ...
Jun Yin +4 more
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Light-sheet fluorescence microscopy for quantitative biology
Nature Methods, 2014In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
Ernst H K Stelzer
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A guide to light-sheet fluorescence microscopy for multiscale imaging
Nature Methods, 2017R. Power, J. Huisken
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Optics Letters, 2023
We present a snapshot temporal compressive light-sheet fluorescence microscopy system to capture high-speed microscopic scenes with a low-speed camera. A deep denoising network and total variation denoiser are incorporated into a plug-and-play framework ...
Meiqin Wang +6 more
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We present a snapshot temporal compressive light-sheet fluorescence microscopy system to capture high-speed microscopic scenes with a low-speed camera. A deep denoising network and total variation denoiser are incorporated into a plug-and-play framework ...
Meiqin Wang +6 more
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Fast Fluorescence Microscopy with Light Sheets
The Biological Bulletin, 2016In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of
Daetwyler, S., Huisken, J.
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Digital Scanned Laser Light Sheet Fluorescence Microscopy
Cold Spring Harbor Protocols, 2010INTRODUCTIONModern applications in the life sciences are frequently based on in vivo imaging of biological specimens, a domain for which light microscopy approaches are typically best suited. Often, quantitative information must be obtained from large multicellular organisms on the cellular or even subcellular level and with a good temporal resolution.
Philipp J, Keller, Ernst H K, Stelzer
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Light-sheet fluorescence microscopy with metalens
ODS 2020: Industrial Optical Devices and Systems, 2020We propose a Light-sheet fluorescent microscopy (LSFM) system with a flat metalens, which is used to replace the bulky illuminating components. The metalens is a diffractive optics elements (DOEs) composed of various gallium nitride (GaN) nanopillar for light-sheet generation, and it can be readily integrated in existing LSFM systems.
Cheng Hung Chu +6 more
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Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
Journal of Visualized Experiments, 2022Deafness is the most common sensory impairment, affecting approximately 5% or 430 million people worldwide as per the World Health Organization1. Aging or presbycusis is a primary cause of sensorineural hearing loss and is characterized by damage to hair cells, spiral ganglion neurons (SGNs), and the stria vascularis. These structures reside within the
Peter A, Santi, Shane B, Johnson
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3D light-sheet fluorescence microscopy in preclinical and clinical drug discovery.
Drug Discovery TodayLight-sheet fluorescence microscopy (LSFM) combined with tissue clearing has emerged as a powerful technology in drug discovery. LSFM is applicable to a variety of samples, from rodent organs to clinical tissue biopsies, and has been used for ...
Urmas Roostalu +2 more
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Journal of Visualized Experiments
Despite the numerous clearing techniques that emerged in the last decade, processing postmortem human brains remains a challenging task due to its dimensions and complexity, which make imaging with micrometer resolution particularly difficult. This paper
Danila Di Meo +8 more
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Despite the numerous clearing techniques that emerged in the last decade, processing postmortem human brains remains a challenging task due to its dimensions and complexity, which make imaging with micrometer resolution particularly difficult. This paper
Danila Di Meo +8 more
semanticscholar +1 more source