Results 1 to 10 of about 38,595 (231)

Light sheet fluorescence microscopy of cleared human eyes [PDF]

Communications Biology, 2023
We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche, Ysé Borella, Anna Verschueren, Ivana Gantar, Stéphane Pagès, Laura Batti, Michel Paques   +6 more
doaj   +4 more sources

Meta-lens light-sheet fluorescence microscopy for in vivo imaging [PDF]

Nanophotonics, 2022
Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan, Tseng Ming Lun, Vyas Sunil, Hsieh Ting-Yu, Wu Jui-Ching, Chen Shang-Yang, Peng Hsiao-Fang, Su Vin-Cent, Huang Tzu-Ting, Kuo Hsin Yu, Chu Cheng Hung, Chen Mu Ku, Chen Jia-Wern, Chen Yu-Chun, Huang Kuang-Yuh, Kuan Chieh-Hsiung, Shi Xu, Misawa Hiroaki, Tsai Din Ping   +18 more
doaj   +6 more sources

Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain [PDF]

Methods and Protocols, 2023
Whole-brain imaging is important for understanding brain functions through deciphering tissue structures, neuronal circuits, and single-neuron tracing.
Youngjae Ryu, Yoonju Kim, Sang-Joon Park, Sung Rae Kim, Hyung-Jun Kim, Chang Man Ha   +5 more
doaj   +2 more sources

In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development [PDF]

Scientific Data, 2022
Design Type(s) non-invasive long-term fluorescence live imaging Measurement Type(s) three-dimensional fluorophore distribution over time in a developing organism Technology Type(s) digital scanned laser light sheet fluorescence microscopy (DSLM) Factor ...
Frederic Strobl, Marc F. Schetelig, Ernst H. K. Stelzer   +2 more
doaj   +2 more sources

A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults [PDF]

Frontiers in Cell and Developmental Biology, 2022
Light sheet fluorescence microscopy (LSFM) has become a method of choice for live imaging because of its fast acquisition and reduced photobleaching and phototoxicity.
Jayson J. Smith, Jayson J. Smith, Jayson J. Smith, Isabel W. Kenny, Isabel W. Kenny, Carsten Wolff, Carsten Wolff, Rachel Cray, Abhishek Kumar, Abhishek Kumar, David R. Sherwood, David R. Sherwood, David Q. Matus, David Q. Matus   +13 more
doaj   +2 more sources

Sidelobe suppression in structured light sheet fluorescence microscopy by the superposition of two light sheets. [PDF]

Biomed Opt Express, 2023
Light sheet microscopy has emerged as a powerful technique for three-dimensional and long-term vivo imaging within neuroscience and developmental biology. A light sheet illumination with structured light fields allows a better tradeoff between the field of view and axial resolution but suffers from strong side lobes.
Han Q, Shi J, Shi F.
europepmc   +3 more sources

Metalens-Based Dual Light-Sheet Fluorescence Microscopy. [PDF]

Small Methods
Abstract Light‐sheet fluorescence microscopy (LSFM) provides optically sectioned fluorescence images with excellent background rejection for rapid and volumetric imaging. However, traditional LSFM typically relies on single‐sided illumination and the stripe artifacts due to partial obstruction or scattering of the illumination beam ...
Luo Y, Chang CC, Hsu HC, Huang BW, Vyas S, Tseng SJ, Chu CH, Tanaka T, Huang KY, Tsai DP.   +9 more
europepmc   +4 more sources

Dynamics of bacterial biofilm development imaged using light sheet fluorescence microscopy [PDF]

Biochemistry and Biophysics Reports
Biofilm formation exacerbates bacterial infections and interferes with industrial processes. However, the dynamics of biofilm development, especially if formed by a combination of more than one species, is not entirely understood.
Lenka Šmerdová, Tibor Füzik, Lucie Valentová, Pavol Bárdy, Michaela Procházková, Martina Pařenicová, Pavel Plevka   +6 more
doaj   +2 more sources

Light Sheet Fluorescence Microscopy [PDF]

Journal of Histochemistry and Cytochemistry, 2011
Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
exaly   +3 more sources

Improved image contrast in nonlinear light-sheet fluorescence microscopy using i $$^2$$ 2 PIE Pulse compression [PDF]

Scientific Reports
Nonlinear microscopy has become an invaluable tool for biological imaging, offering high-resolution visualization of biological specimens. In this manuscript, we present the application of a spectral phase measurement technique, i $$^2$$ 2 PIE, to ...
Imraan Badrodien, Pieter H. Neethling, Gurthwin W. Bosman   +2 more
doaj   +2 more sources