Fifty high-content light sheet fluorescence microscopy datasets of Tribolium castaneum embryogenesis [PDF]
Scientific DataThe red flour beetle (Tribolium castaneum) is a key model organism in developmental biology, genetics, and agricultural research. To address the limited availability of high-quality microscopy data documenting its embryonic morphogenesis, we assembled ...
Frederic Strobl +8 more
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Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging [PDF]
Frontiers in Neuroanatomy, 2021 Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns.
Zhouzhou Zhang +13 more
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Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting [PDF]
Journal of the Royal Society Interface, 2014 Abstract We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section.
Liren Zhu, Chiye Li, Lihong V Wang
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3D Volume Rendering of Invertebrates Using Light-Sheet Fluorescence Microscopy
Photonics, 2022 Light-Sheet Fluorescence Microscopy has recently emerged as the technique of choice for obtaining high quality three-dimensional (3D) images of whole organisms, with low photo-damage and fast acquisition rates.
Emilio J. Gualda
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Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning [PDF]
Intelligent ComputingLight-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Chen Li +4 more
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3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy [PDF]
Communications Biology, 2022 An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Luca Pesce +11 more
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LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy [PDF]
npj ImagingCancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system.
Sabrina M. Lewis +9 more
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Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. [PDF]
PLoS ONE, 2018 Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation.
Savannah L Logan +5 more
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A guide to light-sheet fluorescence microscopy for multiscale imaging
Nature Methods, 2017 The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as developmental and cell biology, anatomical science, biophysics and neuroscience. Although adoption among biologists has been steady, LSFM has not displaced more traditional imaging methods despite its often-superior performance.
Rory M Power, Jan Huisken, Huisken Jan
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In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy [PDF]
Scientific ReportsAutosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent genetic kidney disorder. Animal preclinical studies are one of the main tools to study this disease, often through either 2D histology imaging for high-resolution analysis or CT ...
Pablo Delgado-Rodriguez +10 more
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