Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging [PDF]
Frontiers in Neuroanatomy, 2021 Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns.
Zhouzhou Zhang +13 more
doaj +2 more sources
Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning [PDF]
Intelligent ComputingLight-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Chen Li +4 more
doaj +2 more sources
Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
doaj +5 more sources
Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry & Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light,
openaire +4 more sources
3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy [PDF]
Communications Biology, 2022 An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Luca Pesce +11 more
doaj +2 more sources
Metalens-Based Dual Light-Sheet Fluorescence Microscopy. [PDF]
Small MethodsAbstract Light‐sheet fluorescence microscopy (LSFM) provides optically sectioned fluorescence images with excellent background rejection for rapid and volumetric imaging. However, traditional LSFM typically relies on single‐sided illumination and the stripe artifacts due to partial obstruction or scattering of the illumination beam ...
Luo Y +9 more
europepmc +3 more sources
Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. [PDF]
PLoS ONE, 2018 Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation.
Savannah L Logan +5 more
doaj +4 more sources
Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy [PDF]
Frontiers in Cellular Neuroscience, 2014 The development of three dimensional cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex three ...
Emilio J Gualda +8 more
doaj +4 more sources
LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy [PDF]
npj ImagingCancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system.
Sabrina M. Lewis +9 more
doaj +2 more sources
Light-sheet microscopy: a tutorial [PDF]
Advances in Optics and Photonics, 2018 This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the
Andilla, Jordi +3 more
core +5 more sources