Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. [PDF]
PLoS ONE, 2018 Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation.
Savannah L Logan +5 more
doaj +6 more sources
Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting [PDF]
Journal of the Royal Society Interface, 2014 Abstract We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section.
Lihong V. Wang, Liren Zhu, Chiye Li
exaly +6 more sources
Three-photon light-sheet fluorescence microscopy [PDF]
Optics Letters, 2018 We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
core +6 more sources
Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
doaj +2 more sources
Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry & Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
Peter A. Santi
openaire +3 more sources
Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
doaj +2 more sources
Fifty high-content light sheet fluorescence microscopy datasets of Tribolium castaneum embryogenesis [PDF]
Scientific DataThe red flour beetle (Tribolium castaneum) is a key model organism in developmental biology, genetics, and agricultural research. To address the limited availability of high-quality microscopy data documenting its embryonic morphogenesis, we assembled ...
Frederic Strobl +8 more
doaj +2 more sources
Improved image contrast in nonlinear light-sheet fluorescence microscopy using i $$^2$$ 2 PIE Pulse compression [PDF]
Scientific ReportsNonlinear microscopy has become an invaluable tool for biological imaging, offering high-resolution visualization of biological specimens. In this manuscript, we present the application of a spectral phase measurement technique, i $$^2$$ 2 PIE, to ...
Imraan Badrodien +2 more
doaj +2 more sources
Removing striping artifacts in light-sheet fluorescence microscopy: a review [PDF]
Progress in Biophysics and Molecular Biology, 2022 In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods.
Ricci P. +7 more
core +8 more sources
Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning [PDF]
Intelligent ComputingLight-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Chen Li +4 more
doaj +2 more sources