Results 31 to 40 of about 38,595 (231)

Removing striping artifacts in light-sheet fluorescence microscopy: a review [PDF]

open access: yesProgress in Biophysics and Molecular Biology, 2022
In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods.
Ricci P.   +7 more
openaire   +3 more sources

Light-sheets and smart microscopy, an exciting future is dawning

open access: yesCommunications Biology, 2023
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
doaj   +1 more source

Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces

open access: yesFrontiers in Cell and Developmental Biology, 2021
The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface.
Chad M. Hobson   +3 more
doaj   +1 more source

Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis

open access: yesFrontiers in Plant Science, 2020
Annexin 1 (ANN1) is the most abundant member of the evolutionary conserved multigene protein superfamily of annexins in plants. Generally, annexins participate in diverse cellular processes, such as cell growth, differentiation, vesicle trafficking, and ...
Michaela Tichá   +7 more
doaj   +1 more source

Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope [PDF]

open access: yesEPJ Web of Conferences, 2022
Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets ...
Hofmann Meike   +5 more
doaj   +1 more source

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart [PDF]

open access: yesJournal of Visualized Experiments, 2018
Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain
Yichen, Ding   +8 more
openaire   +2 more sources

Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy

open access: yesCell Reports, 2012
Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok   +5 more
doaj   +3 more sources

Light Sheet Fluorescence Microscopy

open access: yesProceeding of the Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020, 2020
In light-sheet fluorescence microscopy (LSFM) the optical axes of the image-forming microscope objective and the objective providing illumination of the specimen are at right angles to one another (Olarte et al., 2018). A sheet of light is incident on the specimen, and fluorescence is produced and hence detected only in that plane.
openaire   +2 more sources

Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy [PDF]

open access: yesEPJ Web of Conferences, 2023
Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs.
Tortora Giorgia   +5 more
doaj   +1 more source

Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques

open access: yesFrontiers in Neuroanatomy, 2021
The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli   +19 more
doaj   +1 more source

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