Results 31 to 40 of about 5,336,418 (265)

Light sheet fluorescence microscopy for neuroscience [PDF]

Journal of Neuroscience Methods, 2019
The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella, Gunn-Moore, Frank, Dholakia, Kishan   +2 more
openaire   +4 more sources

Light Sheet Fluorescence Microscopy (LSFM) [PDF]

Current Protocols in Cytometry, 2015
Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams, Andrew F, Loftus, Sarah E, Dunn, Matthew S, Joens, James A J, Fitzpatrick   +4 more
openaire   +2 more sources

Light-sheets and smart microscopy, an exciting future is dawning

Communications Biology, 2023
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
doaj   +1 more source

Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]

, 2014
The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Vettenburg, T.; id_orcid, J. Nylk, K. Dholakia, Nylk, J., C. Mitchell, Vettenburg, T., Gunn-Moore, F. J., T. Vettenburg, Mitchell, C., Dholakia, K., Nylk, J.; id_orcid, F. J. Gunn-Moore   +11 more
core   +1 more source

3D volume rendering of invertebrates using light-sheet fluorescence microscopy [PDF]

, 2015
Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates.
Gualda Manzano, Emilio José, Moreno, Nuno   +1 more
core   +1 more source

Confocal microscopy [PDF]

, 2011
Chapter focusing on confocal microscopy. A confocal microscope is one in which the illumination is confined to a small volume in the specimen, the detection is confined to the same volume and the image is built up by scanning this volume over the ...
Wilson, Tony, McConnell, Gail, Amos, William   +2 more
core   +3 more sources

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard, Richard Ankerhold, Drummen, Gregor P. C., Gregor P. C. Drummen, Hellen C. Ishikawa-Ankerhold, Ishikawa-Ankerhold, Hellen C.   +5 more
core   +1 more source

Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces

Frontiers in Cell and Developmental Biology, 2021
The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface.
Chad M. Hobson, Jesse S. Aaron, John M. Heddleston, Teng-Leong Chew   +3 more
doaj   +1 more source

Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis

Frontiers in Plant Science, 2020
Annexin 1 (ANN1) is the most abundant member of the evolutionary conserved multigene protein superfamily of annexins in plants. Generally, annexins participate in diverse cellular processes, such as cell growth, differentiation, vesicle trafficking, and ...
Michaela Tichá, Hendrik Richter, Miroslav Ovečka, Nicola Maghelli, Miroslava Hrbáčková, Petr Dvořák, Jozef Šamaj, Olga Šamajová   +7 more
doaj   +1 more source

Study of dendritic spine compartmentalization : a correlative fluorescence light microscopy-electron microscopy approach [PDF]

, 2014
Neurons communicate with each other through synapses. Most excitatory synapses contact small protrusions called dendritic spines. Spines are connected to dendrites by a very thin stalk called the “spine neck” which restricts diffusion between the spine ...
Cyprien, Vivien
core   +1 more source