Results 31 to 40 of about 38,595 (231)
Removing striping artifacts in light-sheet fluorescence microscopy: a review [PDF]
In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods.
Ricci P. +7 more
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Light-sheets and smart microscopy, an exciting future is dawning
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
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Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces
The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface.
Chad M. Hobson +3 more
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Annexin 1 (ANN1) is the most abundant member of the evolutionary conserved multigene protein superfamily of annexins in plants. Generally, annexins participate in diverse cellular processes, such as cell growth, differentiation, vesicle trafficking, and ...
Michaela Tichá +7 more
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Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope [PDF]
Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets ...
Hofmann Meike +5 more
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Light-sheet Fluorescence Microscopy for the Study of the Murine Heart [PDF]
Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain
Yichen, Ding +8 more
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Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy
Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok +5 more
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Light Sheet Fluorescence Microscopy
In light-sheet fluorescence microscopy (LSFM) the optical axes of the image-forming microscope objective and the objective providing illumination of the specimen are at right angles to one another (Olarte et al., 2018). A sheet of light is incident on the specimen, and fluorescence is produced and hence detected only in that plane.
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Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy [PDF]
Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs.
Tortora Giorgia +5 more
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The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli +19 more
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