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Light sheet fluorescence microscopy of cleared human eyes [PDF]

Communications Biology, 2023
We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche, Ysé Borella, Anna Verschueren, Ivana Gantar, Stéphane Pagès, Laura Batti, Michel Paques   +6 more
doaj   +5 more sources

Meta-lens light-sheet fluorescence microscopy for in vivo imaging [PDF]

Nanophotonics, 2022
Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan, Tseng Ming Lun, Vyas Sunil, Hsieh Ting-Yu, Wu Jui-Ching, Chen Shang-Yang, Peng Hsiao-Fang, Su Vin-Cent, Huang Tzu-Ting, Kuo Hsin Yu, Chu Cheng Hung, Chen Mu Ku, Chen Jia-Wern, Chen Yu-Chun, Huang Kuang-Yuh, Kuan Chieh-Hsiung, Shi Xu, Misawa Hiroaki, Tsai Din Ping   +18 more
doaj   +3 more sources

Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain [PDF]

Methods and Protocols, 2023
Whole-brain imaging is important for understanding brain functions through deciphering tissue structures, neuronal circuits, and single-neuron tracing.
Youngjae Ryu, Yoonju Kim, Sang-Joon Park, Sung Rae Kim, Hyung-Jun Kim, Chang Man Ha   +5 more
doaj   +3 more sources

Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning [PDF]

Intelligent Computing
Light-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Chen Li, Mani Ratnam Rai, Yuheng Cai, H. Troy Ghashghaei, Alon Greenbaum   +4 more
doaj   +3 more sources

Dynamics of bacterial biofilm development imaged using light sheet fluorescence microscopy [PDF]

Biochemistry and Biophysics Reports
Biofilm formation exacerbates bacterial infections and interferes with industrial processes. However, the dynamics of biofilm development, especially if formed by a combination of more than one species, is not entirely understood.
Lenka Šmerdová, Tibor Füzik, Lucie Valentová, Pavol Bárdy, Michaela Procházková, Martina Pařenicová, Pavel Plevka   +6 more
doaj   +3 more sources

Multiple airy beams light-sheet fluorescence microscopy

Frontiers in Physics, 2022
Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu, Shuangyu Gu, Xianghua Yu, Xianghua Yu, Chen Bai, Junwei Min, Runze Li, Yanlong Yang, Baoli Yao, Baoli Yao   +9 more
doaj   +3 more sources

In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development [PDF]

Scientific Data, 2022
Design Type(s) non-invasive long-term fluorescence live imaging Measurement Type(s) three-dimensional fluorophore distribution over time in a developing organism Technology Type(s) digital scanned laser light sheet fluorescence microscopy (DSLM) Factor ...
Frederic Strobl, Marc F. Schetelig, Ernst H. K. Stelzer   +2 more
doaj   +3 more sources

A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults [PDF]

Frontiers in Cell and Developmental Biology, 2022
Light sheet fluorescence microscopy (LSFM) has become a method of choice for live imaging because of its fast acquisition and reduced photobleaching and phototoxicity.
Jayson J. Smith, Jayson J. Smith, Jayson J. Smith, Isabel W. Kenny, Isabel W. Kenny, Carsten Wolff, Carsten Wolff, Rachel Cray, Abhishek Kumar, Abhishek Kumar, David R. Sherwood, David R. Sherwood, David Q. Matus, David Q. Matus   +13 more
doaj   +3 more sources

3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy [PDF]

Communications Biology, 2022
An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Luca Pesce, Marina Scardigli, Vladislav Gavryusev, Annunziatina Laurino, Giacomo Mazzamuto, Niamh Brady, Giuseppe Sancataldo, Ludovico Silvestri, Christophe Destrieux, Patrick R. Hof, Irene Costantini, Francesco S. Pavone   +11 more
doaj   +3 more sources

Myelinated fiber labeling and orientation mapping of the human brain with light-sheet fluorescence microscopy. [PDF]

Neuroimage
The convoluted network of myelinated fibers that supports behavior, cognition, and sensory processing in the human brain is the source of its extraordinary complexity.
Sorelli M, Di Meo D, Bradley S, Cheli F, Ramazzotti J, Caria FF, Perego L, Destrieux C, Hof PR, Pavone FS, Mazzamuto G, Costantini I.   +11 more
europepmc   +2 more sources