In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy [PDF]
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent genetic kidney disorder. Animal preclinical studies are one of the main tools to study this disease, often through either 2D histology imaging for high-resolution analysis or CT ...
Pablo Delgado-Rodriguez +10 more
doaj +2 more sources
Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy
The development of three dimensional cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex three ...
Emilio J Gualda +8 more
doaj +3 more sources
Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids [PDF]
Oksana Netschitailo +8 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
doaj +1 more source
Multiple airy beams light-sheet fluorescence microscopy
Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu +9 more
doaj +1 more source
Innovations in ex vivo Light Sheet Fluorescence Microscopy [PDF]
Light Sheet Fluorescence Microscopy (LSFM) has revolutionized how optical imaging of biological specimens can be performed as this technique allows to produce 3D fluorescence images of entire samples with a high spatiotemporal resolution. In this manuscript, we aim to provide readers with an overview of the field of LSFM on ex vivo samples.
Pablo Delgado-Rodriguez +3 more
openaire +3 more sources
Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
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Light sheet fluorescence microscopy for neuroscience [PDF]
The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella +2 more
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Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
doaj +1 more source
Three-photon light-sheet fluorescence microscopy [PDF]
We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
openaire +4 more sources

