Improved image contrast in nonlinear light-sheet fluorescence microscopy using i 2 PIE Pulse compression. [PDF]
Sci RepNonlinear microscopy has become an invaluable tool for biological imaging, offering high-resolution visualization of biological specimens. In this manuscript, we present the application of a spectral phase measurement technique, i $$^2$$ 2 PIE, to ...
Badrodien I, Neethling PH, Bosman GW.
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Innovations in ex vivo Light Sheet Fluorescence Microscopy [PDF]
Progress in Biophysics and Molecular Biology, 2022 Light Sheet Fluorescence Microscopy (LSFM) has revolutionized how optical imaging of biological specimens can be performed as this technique allows to produce 3D fluorescence images of entire samples with a high spatiotemporal resolution. In this manuscript, we aim to provide readers with an overview of the field of LSFM on ex vivo samples.
Pablo Delgado-Rodriguez +3 more
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Light sheet fluorescence microscopy for neuroscience [PDF]
Journal of Neuroscience Methods, 2019 The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella +2 more
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Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Current Protocols in Cytometry, 2015 Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
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Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning. [PDF]
Intell ComputLight-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Li C +4 more
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Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging. [PDF]
Front Neuroanat, 2021 Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns.
Zhang Z +8 more
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Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
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Three-photon light-sheet fluorescence microscopy [PDF]
Optics Letters, 2018 We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
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Light-sheets and smart microscopy, an exciting future is dawning
Communications Biology, 2023 Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
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Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids. [PDF]
Biol OpenNetschitailo O +8 more
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