Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy. [PDF]
J Cell Biol, 2023 Delage et al. provide a primer on light sheet fluorescence microscopy to guide biologists through the specific challenges of this 3D imaging technique.
Delage E, Guilbert T, Yates F.
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Deep learning-based adaptive optics for light sheet fluorescence microscopy. [PDF]
Biomed Opt Express, 2023 Light sheet fluorescence microscopy (LSFM) is a high-speed imaging technique that is often used to image intact tissue-cleared specimens with cellular or subcellular resolution. Like other optical imaging systems, LSFM suffers from sample-induced optical
Rai MR +3 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter [PDF]
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Deep learning enhanced light sheet fluorescence microscopy for in vivo 4D imaging of zebrafish heart beating. [PDF]
Light Sci ApplTime-resolved volumetric fluorescence imaging over an extended duration with high spatial/temporal resolution is a key driving force in biomedical research for investigating spatial-temporal dynamics at organism-level systems, yet it remains a major ...
Zhang M +6 more
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Integrating 4-D light-sheet fluorescence microscopy and genetic zebrafish system to investigate ambient pollutants-mediated toxicity. [PDF]
Sci Total Environ, 2023 Ambient air pollutants, including PM2.5 (aerodynamic diameter d ~2.5 μm), PM10 (d ~10 μm), and ultrafine particles (UFP: d
Gonzalez-Ramos S +9 more
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Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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Multimodal optical coherence tomography and two-photon light sheet fluorescence microscopy for embryo imaging. [PDF]
J Biomed Opt. Significance Structural and molecular imaging of the developing embryo can provide deep insights into the development of various pathologies, but few techniques enable the simultaneous detection of these parameters.
Karim MM +8 more
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Removing striping artifacts in light-sheet fluorescence microscopy: A review. [PDF]
Progress in Biophysics and Molecular Biology, 2021 In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to
Pietro Ricci +7 more
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Remote-refocusing light-sheet fluorescence microscopy enables 3D imaging of electromechanical coupling of hiPSC-derived and adult cardiomyocytes in co-culture [PDF]
Sci Rep, 2023 Improving cardiac function through stem-cell regenerative therapy requires functional and structural integration of the transplanted cells with the host tissue.
Dvinskikh L +5 more
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High-speed 2D light-sheet fluorescence microscopy enables quantification of spatially varying calcium dynamics in ventricular cardiomyocytes. [PDF]
Front Physiol, 2023 Introduction: Reduced synchrony of calcium release and t-tubule structure organization in individual cardiomyocytes has been linked to loss of contractile strength and arrhythmia.
Dvinskikh L +3 more
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