Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning [PDF]
Intelligent ComputingLight-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Chen Li +4 more
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3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy [PDF]
Communications Biology, 2022 An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Luca Pesce +11 more
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LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy [PDF]
npj ImagingCancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system.
Sabrina M. Lewis +9 more
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Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. [PDF]
PLoS ONE, 2018 Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation.
Savannah L Logan +5 more
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In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy [PDF]
Scientific ReportsAutosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent genetic kidney disorder. Animal preclinical studies are one of the main tools to study this disease, often through either 2D histology imaging for high-resolution analysis or CT ...
Pablo Delgado-Rodriguez +10 more
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Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids [PDF]
Biology OpenOksana Netschitailo +8 more
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Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array. [PDF]
Laser Photon Rev, 2022 Cai Y +5 more
europepmc +2 more sources
Sidelobe suppression in structured light sheet fluorescence microscopy by the superposition of two light sheets. [PDF]
Biomed Opt Express, 2023 Light sheet microscopy has emerged as a powerful technique for three-dimensional and long-term vivo imaging within neuroscience and developmental biology. A light sheet illumination with structured light fields allows a better tradeoff between the field of view and axial resolution but suffers from strong side lobes.
Han Q, Shi J, Shi F.
europepmc +3 more sources
Metalens-Based Dual Light-Sheet Fluorescence Microscopy. [PDF]
Small MethodsAbstract Light‐sheet fluorescence microscopy (LSFM) provides optically sectioned fluorescence images with excellent background rejection for rapid and volumetric imaging. However, traditional LSFM typically relies on single‐sided illumination and the stripe artifacts due to partial obstruction or scattering of the illumination beam ...
Luo Y +9 more
europepmc +4 more sources