Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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Fifty high-content light sheet fluorescence microscopy datasets of Tribolium castaneum embryogenesis [PDF]
Scientific DataThe red flour beetle (Tribolium castaneum) is a key model organism in developmental biology, genetics, and agricultural research. To address the limited availability of high-quality microscopy data documenting its embryonic morphogenesis, we assembled ...
Frederic Strobl +8 more
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Improved image contrast in nonlinear light-sheet fluorescence microscopy using i $$^2$$ 2 PIE Pulse compression [PDF]
Scientific ReportsNonlinear microscopy has become an invaluable tool for biological imaging, offering high-resolution visualization of biological specimens. In this manuscript, we present the application of a spectral phase measurement technique, i $$^2$$ 2 PIE, to ...
Imraan Badrodien +2 more
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Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging [PDF]
Frontiers in Neuroanatomy, 2021 Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns.
Zhouzhou Zhang +13 more
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Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning [PDF]
Intelligent ComputingLight-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Chen Li +4 more
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Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting [PDF]
Journal of the Royal Society Interface, 2014 Abstract We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section.
Lihong Wang, Liren Zhu, Chiye Li
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Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry and Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
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3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy [PDF]
Communications Biology, 2022 An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Luca Pesce +11 more
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LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy [PDF]
npj ImagingCancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system.
Sabrina M. Lewis +9 more
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A guide to light-sheet fluorescence microscopy for multiscale imaging
Nature Methods, 2017 The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as developmental and cell biology, anatomical science, biophysics and neuroscience. Although adoption among biologists has been steady, LSFM has not displaced more traditional imaging methods despite its often-superior performance.
Jan Huisken, Huisken Jan
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