Results 31 to 40 of about 40,411 (233)

Removing striping artifacts in light-sheet fluorescence microscopy: a review [PDF]

Progress in Biophysics and Molecular Biology, 2022
In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods.
Ricci P., Gavryusev V., Mullenbroich C., Turrini L., de Vito G., Silvestri L., Sancataldo G., Pavone F. S.   +7 more
openaire   +4 more sources

3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy. [PDF]

Commun Biol, 2022
An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Pesce L, Scardigli M, Gavryusev V, Laurino A, Mazzamuto G, Brady N, Sancataldo G, Silvestri L, Destrieux C, Hof PR, Costantini I, Pavone FS.   +11 more
europepmc   +2 more sources

LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy. [PDF]

Npj Imaging
Cancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system.
Lewis SM, Berthelet J, Whitehead LW, Rajasekhar P, El-Saafin F, Bell C, Naik S, Merino D, Wimmer VC, Rogers KL.   +9 more
europepmc   +2 more sources

Light Sheet Fluorescence Microscopy [PDF]

Journal of Histochemistry & Cytochemistry, 2011
Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
openaire   +2 more sources

Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces

Frontiers in Cell and Developmental Biology, 2021
The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface.
Chad M. Hobson, Jesse S. Aaron, John M. Heddleston, Teng-Leong Chew   +3 more
doaj   +1 more source

Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis

Frontiers in Plant Science, 2020
Annexin 1 (ANN1) is the most abundant member of the evolutionary conserved multigene protein superfamily of annexins in plants. Generally, annexins participate in diverse cellular processes, such as cell growth, differentiation, vesicle trafficking, and ...
Michaela Tichá, Hendrik Richter, Miroslav Ovečka, Nicola Maghelli, Miroslava Hrbáčková, Petr Dvořák, Jozef Šamaj, Olga Šamajová   +7 more
doaj   +1 more source

Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope [PDF]

EPJ Web of Conferences, 2022
Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets ...
Hofmann Meike, Gharbi Ghebjagh Shima, Fan Chao, Feng Yuchao, Lemke Karen, Sinzinger Stefan   +5 more
doaj   +1 more source

Light Sheet Fluorescence Microscopy

Proceeding of the Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020, 2020
In light-sheet fluorescence microscopy (LSFM) the optical axes of the image-forming microscope objective and the objective providing illumination of the specimen are at right angles to one another (Olarte et al., 2018). A sheet of light is incident on the specimen, and fluorescence is produced and hence detected only in that plane.
openaire   +2 more sources

In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy. [PDF]

Sci Rep
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent genetic kidney disorder. Animal preclinical studies are one of the main tools to study this disease, often through either 2D histology imaging for high-resolution analysis or CT ...
Delgado-Rodriguez P, Vitoria I, Ríos-Muñoz GR, Bardia L, Lamanna-Rama N, Nicolas-Saenz L, Sporring J, Soto-Montenegro ML, Aldabe R, Colombelli J, Muñoz-Barrutia A.   +10 more
europepmc   +2 more sources

Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser

Methods and Protocols, 2019
Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just ...
Diego Di Battista, David Merino, Giannis Zacharakis, Pablo Loza-Alvarez, Omar E. Olarte   +4 more
doaj   +1 more source