3D Volume Rendering of Invertebrates Using Light-Sheet Fluorescence Microscopy
Photonics, 2022 Light-Sheet Fluorescence Microscopy has recently emerged as the technique of choice for obtaining high quality three-dimensional (3D) images of whole organisms, with low photo-damage and fast acquisition rates.
Emilio J. Gualda
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Elimination of stripe artifacts in light sheet fluorescence microscopy using an attention-based residual neural network. [PDF]
Biomed Opt Express, 2022 Stripe artifacts can deteriorate the quality of light sheet fluorescence microscopy (LSFM) images. Owing to the inhomogeneous, high-absorption, or scattering objects located in the excitation light path, stripe artifacts are generated in LSFM images in ...
Wei Z +6 more
europepmc +2 more sources
Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
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Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry & Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light,
openaire +4 more sources
Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy. [PDF]
Biomed Opt Express, 2021 Light-sheet fluorescence microscopy (LSFM) is a minimally invasive and high throughput imaging technique ideal for capturing large volumes of tissue with sub-cellular resolution. A fundamental requirement for LSFM is a seamless overlap of the light-sheet
Li C +4 more
europepmc +2 more sources
Digital Brain Maps and Virtual Neuroscience: An Emerging Role for Light-Sheet Fluorescence Microscopy in Drug Development. [PDF]
Front Neurosci, 2022 The mammalian brain is by far the most advanced organ to have evolved and the underlying biology is extremely complex. However, with aging populations and sedentary lifestyles, the prevalence of neurological disorders is increasing around the world ...
Perens J, Hecksher-Sørensen J.
europepmc +2 more sources
Metalens-Based Dual Light-Sheet Fluorescence Microscopy. [PDF]
Small MethodsAbstract Light‐sheet fluorescence microscopy (LSFM) provides optically sectioned fluorescence images with excellent background rejection for rapid and volumetric imaging. However, traditional LSFM typically relies on single‐sided illumination and the stripe artifacts due to partial obstruction or scattering of the illumination beam ...
Luo Y +9 more
europepmc +3 more sources
Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. [PDF]
PLoS ONE, 2018 Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation.
Savannah L Logan +5 more
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Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy [PDF]
Frontiers in Cellular Neuroscience, 2014 The development of three dimensional cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex three ...
Emilio J Gualda +8 more
doaj +4 more sources
LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy [PDF]
npj ImagingCancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system.
Sabrina M. Lewis +9 more
doaj +2 more sources