Three-photon light-sheet fluorescence microscopy [PDF]
Optics Letters, 2018 We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
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Planar Airy beam light-sheet for two-photon microscopy [PDF]
, 2020 We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-
Almada, Pedro +12 more
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Light-sheets and smart microscopy, an exciting future is dawning
Communications Biology, 2023 Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
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Light sheet fluorescence microscopy for neuroscience [PDF]
Journal of Neuroscience Methods, 2019 The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella +2 more
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Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]
, 2014 The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Dholakia, K. +4 more
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Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes. [PDF]
, 2016 The conventional optical microscope is an inherently two-dimensional (2D) imaging tool. The objective lens, eyepiece and image sensor are all designed to capture light emitted from a 2D 'object plane'.
Bakowski, Malina A +6 more
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Photoswitching-Enabled Contrast Enhancement in Light Sheet Fluorescence Microscopy [PDF]
, 2017 Light sheet fluorescence microscopy enables high-resolution imaging of thick biological samples. By restricting the fluorescence excitation to a single plane, rapid wide-field image acquisition is possible with minimal sample exposure.
Corral, Angélica +4 more
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Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces
Frontiers in Cell and Developmental Biology, 2021 The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface.
Chad M. Hobson +3 more
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Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping. [PDF]
, 2016 Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging ...
Chen, Harrison +12 more
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Single Molecule Imaging of Transcription Factor Binding to DNA in Live Mammalian Cells [PDF]
, 2013 Imaging single fluorescent proteins in living mammalian cells is challenged by out-of-focus fluorescence excitation. To reduce out-of-focus fluorescence we developed reflected light-sheet microscopy (RLSM), a fluorescence microscopy method allowing ...
Basu, Srinjan +7 more
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