Results 51 to 60 of about 38,654 (228)

Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics

npj Imaging
Single-molecule localization microscopy has revealed cellular architectures and molecular dynamics beyond the diffraction limit of light. However, imaging thick samples presents challenges from increased fluorescence background. Light sheet illumination,
Siyang Cheng, Yuya Nakatani, Gabriella Gagliano, Nahima Saliba, Anna-Karin Gustavsson   +4 more
doaj   +1 more source

Patient‐Derived 3D Heart‐On‐a‐Chip Model of Dilated Cardiomyopathy With Embedded Bead‐Based Mapping of Tissue Contractility

Advanced Healthcare Materials, EarlyView.
A heart‐on‐a‐chip model of dilated cardiomyopathy is developed from patient‐derived induced pluripotent stem cells. The model recapitulates key disease phenotypes and enables functional assessment through integrated bead‐based tracking and pillar deflection measurements.
Ali Mousavi, Ludovic Mouttet, Shihao Cui, Yasaman Hekmatnia, Mehran Mottahedi, Ida Derish, Naimeh Rafatian, Mark Aurousseau, Gregor Andelfinger, Renzo Cecere, Houman Savoji   +10 more
wiley   +1 more source

Thin-sheet laser imaging microscopy for optical sectioning of thick tissues

BioTechniques, 2009
We report the development of a modular and optimized thin-sheet laser imaging microscope (TSLIM) for nondestructive optical sectioning of organisms and thick tissues such as the mouse cochlea, zebrafish brain/inner ear, and rat brain at a resolution that
Peter A. Santi, Shane B. Johnson, Matthias Hillenbrand, Patrick Z. Grandpre, Tiffany J. Glass, James R. Leger   +5 more
doaj   +1 more source

Conjugated Porphyrin Polymer With Superior Charge Transfer for Photodynamic‐Immunomodulatory Prevention of CIED Infection in Diabetes

Advanced Healthcare Materials, EarlyView.
Under NIR irradiation, PTCPP generates ROS to eliminate MRSA biofilms and promote M1 macrophage polarization for phagocytosis. After bacterial clearance, the material is phagocytosed by macrophages, shifting their polarization to M2 phenotype via metabolic reprogramming, which synergistically enhances anti‐infection therapy and wound healing.
Honghao Ma, Jinguo Xu, Rongrong Gu, Chuanhui Dong, Te Chen, Yi Mu, Jing Zhu, Yating Wang, Wen Wang, Yingying Zhang, Hongyu Ma, Xiaochen Wang, Sheng Ye, Cheng Cheng   +13 more
wiley   +1 more source

Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM [PDF]

Light: Science & Applications, 2018
AbstractLight sheet fluorescence microscopy (LSFM) is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs. However, LSFM can suffer from optical artifacts for which there is no intrinsic solution.
Mayer, Jürgen, 1977-, Robert Moreno, Alexandre, Sharpe, James, Swoger, Jim   +3 more
openaire   +3 more sources

Multicomponent Peptide‐MXene‐Hyaluronic Acid Hydrogel as a Conductive Scaffold for Enhanced Fibroblast Proliferation and Migration Under Electrical Stimulation

Advanced Healthcare Materials, EarlyView.
An electrically conductive FmocFF/MXene/hyaluronic acid hydrogel combines extracellular matrix‐like architecture, self‐healing behavior, oxidation‐resistant conductivity, and biochemical cues in a single scaffold. By enabling physiologically relevant electrical stimulation, the platform enhances fibroblast attachment, proliferation, and migration ...
Offir Loboda, Dana Cohen‐Gerassi, Dor Aaron Goldstein, Or Messer, Bar Favelukis, Ehud Gazit, Maxim Sokol, Lihi Adler‐Abramovich   +7 more
wiley   +1 more source

Beam shaping in light-sheet microscopy: an experimental analysis

JPhys Photonics
Thanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution.
Manuel Hüpfel, Gerd Ulrich Nienhaus
doaj   +1 more source

Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

eLife, 2020
As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models.
Claudia Prahst, Parham Ashrafzadeh, Thomas Mead, Ana Figueiredo, Karen Chang, Douglas Richardson, Lakshmi Venkaraman, Mark Richards, Ana Martins Russo, Kyle Harrington, Marie Ouarné, Andreia Pena, Dong Feng Chen, Lena Claesson-Welsh, Kin-Sang Cho, Claudio A Franco, Katie Bentley   +16 more
doaj   +1 more source

Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae

Scientific Reports, 2021
Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy.
Dimitrios Kapsokalyvas, Rodrigo Rosas, Rob W. A. Janssen, Jo M. Vanoevelen, Miranda Nabben, Martin Strauch, Dorit Merhof, Marc A. M. J. van Zandvoort   +7 more
doaj   +1 more source

Recent progress of second near-infrared (NIR-II) fluorescence microscopy in bioimaging

Frontiers in Physiology, 2023
Visualizing biological tissues in vivo at a cellular or subcellular resolution to explore molecular signaling and cell behaviors is a crucial direction for research into biological processes.
Tian Wang, Yingying Chen, Bo Wang, Mingfu Wu   +3 more
doaj   +1 more source