Results 61 to 70 of about 136,404 (276)

Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis

open access: yesFrontiers in Plant Science, 2020
Annexin 1 (ANN1) is the most abundant member of the evolutionary conserved multigene protein superfamily of annexins in plants. Generally, annexins participate in diverse cellular processes, such as cell growth, differentiation, vesicle trafficking, and ...
Michaela Tichá   +7 more
doaj   +1 more source

Anatomy of the complete mouse eye vasculature explored by light-sheet fluorescence microscopy exposes subvascular-specific remodeling in development and pathology.

open access: yesExperimental Eye Research, 2023
Eye development and function rely on precise establishment, regression and maintenance of its many sub-vasculatures. These crucial vascular properties have been extensively investigated in eye development and disease utilizing genetic and experimental ...
Luc Krimpenfort   +9 more
semanticscholar   +1 more source

Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope [PDF]

open access: yesEPJ Web of Conferences, 2022
Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets ...
Hofmann Meike   +5 more
doaj   +1 more source

Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy [PDF]

open access: yes, 2020
In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes.
Bainar, Petr   +8 more
core   +1 more source

Light Sheet Fluorescence Microscopy

open access: yesProceeding of the Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020, 2020
In light-sheet fluorescence microscopy (LSFM) the optical axes of the image-forming microscope objective and the objective providing illumination of the specimen are at right angles to one another (Olarte et al., 2018). A sheet of light is incident on the specimen, and fluorescence is produced and hence detected only in that plane.
openaire   +2 more sources

Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting [PDF]

open access: yesJournal of The Royal Society Interface, 2014
We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section.
Gao, Liang   +3 more
openaire   +3 more sources

Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques

open access: yesFrontiers in Neuroanatomy, 2021
The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli   +19 more
doaj   +1 more source

Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy [PDF]

open access: yesEPJ Web of Conferences, 2023
Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs.
Tortora Giorgia   +5 more
doaj   +1 more source

Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy

open access: yesCell Reports, 2012
Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok   +5 more
doaj   +3 more sources

Bessel beam illumination reduces random and systematic errors in quantitative functional studies using light-sheet microscopy [PDF]

open access: yes, 2018
Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a choice method to observe brain function with high frame rates at cellular resolution.
Alterini, Tommaso   +8 more
core   +15 more sources

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