Results 61 to 70 of about 38,595 (230)

Shaping Matter with Acoustic Waves: From Particles to Functional Structures

Advanced Functional Materials, EarlyView.
Acoustic‐directed assembly is reviewed as a materials‐oriented strategy for organizing particles, polymers, metals, and biological systems into functional structures. The review distinguishes reversible manipulation from permanent structure formation through medium fixation, particle fixation, and in situ material generation, highlighting routes toward
Avraham Kenigsberg, Shelly Zlotnikov, Maya Fiorentino, Yuval Elias, Ornit Nagler‐Avramovitz, Hagay Shpaisman   +5 more
wiley   +1 more source

Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain

Frontiers in Neuroanatomy, 2019
Expansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience.
Daniel Normen Düring, Daniel Normen Düring, Daniel Normen Düring, Mariana Diales Rocha, Falk Dittrich, Manfred Gahr, Richard Hans Robert Hahnloser, Richard Hans Robert Hahnloser   +7 more
doaj   +1 more source

Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT [PDF]

Proceedings of the National Academy of Sciences, 2016
Significance Light-sheet fluorescence microscopy (LSFM) is an imaging modality in which a sample is illuminated from the side by a beam engineered into a wide and relatively thin “sheet.” This allows highly parallelized planewise scanning of volumes with inherent optical sectioning, offering a good balance between spatial and temporal ...
Hoyer, P., de Medeiros, G., Balazs, B., Norlin, N., Besir, C., Hanne, J., Kräusslich, H., Engelhardt, J., Sahl, S., Hell, S. ; https://orcid.org/0000-0002-9638-5077, Hufnagel, L.   +10 more
openaire   +4 more sources

Coagulative Granular Hydrogels with an Enzyme Catalyzed Fibrin Network for Endogenous Tissue Regeneration

Advanced Healthcare Materials, EarlyView.
Coagulative granular hydrogels are composed of packed thrombin‐functionalized microgels that catalyze the conversion of fibrinogen into a secondary fibrin network, filling the interstitial voids. This bio‐inspired approach stabilizes the biomaterial to match the robustness of bulk hydrogels without compromising injectability, mimicking the initial ...
Zhipeng Deng, Camila B. Tovani, Simona Bianco, Gianni Comandini, Aya Elghajiji, Mina Aleemardani, Roxana Moscalu, Jeremie Zappia, Bianca Fernandes, Olivia Annett, Chrissy L. Hammond, Jason Wong, Dave J. Adams, Fabrizio Scarpa, Michael R. Whitehouse, Annela M. Seddon, James P. K. Armstrong   +16 more
wiley   +1 more source

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

eLife, 2020
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral
Etai Sapoznik, Bo-Jui Chang, Jaewon Huh, Robert J Ju, Evgenia V Azarova, Theresa Pohlkamp, Erik S Welf, David Broadbent, Alexandre F Carisey, Samantha J Stehbens, Kyung-Min Lee, Arnaldo Marín, Ariella B Hanker, Jens C Schmidt, Carlos L Arteaga, Bin Yang, Yoshihiko Kobayashi, Purushothama Rao Tata, Rory Kruithoff, Konstantin Doubrovinski, Douglas P Shepherd, Alfred Millett-Sikking, Andrew G York, Kevin M Dean, Reto P Fiolka   +24 more
doaj   +1 more source

Shadow imaging for panoptical visualization of brain tissue in vivo

Nature Communications, 2023
Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation (electron microscopy), do not have cellular ...
Yulia Dembitskaya, Andrew K. J. Boyce, Agata Idziak, Atefeh Pourkhalili Langeroudi, Misa Arizono, Jordan Girard, Guillaume Le Bourdellès, Mathieu Ducros, Marie Sato-Fitoussi, Amaia Ochoa de Amezaga, Kristell Oizel, Stephane Bancelin, Luc Mercier, Thomas Pfeiffer, Roger J. Thompson, Sun Kwang Kim, Andreas Bikfalvi, U. Valentin Nägerl   +17 more
doaj   +1 more source

Photothermal‐Activated Antibacterial Amyloid‐Polyphenol‐Iron Hydrogels for Synergistic Wound Healing

Advanced Healthcare Materials, EarlyView.
We report a thermally triggered supramolecular hydrogel (LTFe) formed by Fe3+ and tannic acid on lysozyme amyloid fibrils. Fe3+ enables rapid gelation and photothermal transduction with an efficiency of 88.56%. The LTFe hydrogel exhibits excellent biocompatibility, potent antibacterial activity against E. coli and S.
Di Wu, Jiangtao Zhou, Yang Shen, Qiyao Sun, Tong Li, Xiaoyang Zou, Bin Liu, Hongshan Liang, Raffaele Mezzenga   +8 more
wiley   +1 more source

Keeping Cells Alive in Microscopy

Biophysica
Light microscopy has emerged as one of the fundamental methods to analyze biological systems; novel techniques of 3D microscopy and super-resolution microscopy (SRM) with an optical resolution down to the sub-nanometer range have recently been realized ...
Herbert Schneckenburger, Christoph Cremer   +1 more
doaj   +1 more source

Spontaneous Helical Alignment of Smooth Muscle Cells to Form a Medial Layer for Engineered Microvasculature

Advanced Healthcare Materials, EarlyView.
Spontaneous helical alignment of smooth muscle cells is induced within resistance‐vessel‐sized channels patterned within a hydrogel. The extent of the cells’ orientation angle is dependent on the presence and composition of ECM proteins lining the channel wall and cell seeding density.
Victoria D. Vest, Katherine J. Young, Isabella K. Holtz, Mark Mc Veigh, James D. West, Leon M. Bellan   +5 more
wiley   +1 more source

A Systematic Study of GelMA‐Carbopol Bioinks for High‐Fidelity Extrusion 3D Bioprinting at Physiological Temperatures

Advanced Healthcare Materials, EarlyView.
Gonzalez Martinez and collaborators develop a strategy to formulate high performance GelMA‐based bioinks with low solids contents. The resulting bioinks enable 3D bioprinting at 37 °C of high‐fidelity structures with tunable mechanical properties that support high cell viability and function.
David A. González‐Martínez, Aidee V. Arizpe Tafoya, Eduardo González‐Martínez, Mabel Barreiro Carpio, Laura Daniela Serrano Andrade, Christopher T. N. Chow, Mohammadhossein Dabaghi, Jeremy A. Hirota, Jose M. Moran‐Mirabal   +8 more
wiley   +1 more source