Results 41 to 50 of about 40,411 (233)

Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy

Cell Reports, 2012
Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok, Inna Schwarz, Julia Ledderose, Günter Giese, Karl-Klaus Conzelmann, Martin K. Schwarz   +5 more
doaj   +3 more sources

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart [PDF]

Journal of Visualized Experiments, 2018
Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain
Yichen, Ding, Zachary, Bailey, Victoria, Messerschmidt, Jun, Nie, Richard, Bryant, Sandra, Rugonyi, Peng, Fei, Juhyun, Lee, Tzung K, Hsiai   +8 more
openaire   +2 more sources

Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques

Frontiers in Neuroanatomy, 2021
The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli, Marina Scardigli, Luca Pesce, Luca Pesce, Niamh Brady, Giacomo Mazzamuto, Giacomo Mazzamuto, Vladislav Gavryusev, Vladislav Gavryusev, Ludovico Silvestri, Ludovico Silvestri, Ludovico Silvestri, Patrick R. Hof, Christophe Destrieux, Irene Costantini, Irene Costantini, Irene Costantini, Francesco S. Pavone, Francesco S. Pavone, Francesco S. Pavone   +19 more
doaj   +1 more source

The VHL tumor suppressor at the crossroad of protein folding, aggregation, and cancer

Molecular Oncology, EarlyView.
Mutations, environmental stress, and chaperone dysfunction can destabilize pVHL, promoting its conversion from the native folded state into amyloid‐like assemblies. This transition may contribute to protein storage, cell dormancy, survival, and drug resistance.
Lara Abad, Silvio Carlo Ermanno Tosatto, Emanuela Leonardi   +2 more
wiley   +1 more source

Deformation Measurements of Neuronal Excitability Using Incoherent Holography Lattice Light-Sheet Microscopy (IHLLS)

Photonics, 2021
Stimuli to excitable cells and various cellular processes can cause cell surface deformations; for example, when excitable cell membrane potentials are altered during action potentials.
Mariana Potcoava, Jonathan Art, Simon Alford, Christopher Mann   +3 more
doaj   +1 more source

Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM [PDF]

Light: Science & Applications, 2018
AbstractLight sheet fluorescence microscopy (LSFM) is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs. However, LSFM can suffer from optical artifacts for which there is no intrinsic solution.
Mayer, Jürgen, 1977-, Robert Moreno, Alexandre, Sharpe, James, Swoger, Jim   +3 more
openaire   +3 more sources

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Morphological and Calcium Signaling Alterations of Neuroglial Cells in Cerebellar Cortical Dysplasia Induced by Carmustine

Cells, 2021
Cortical dysplasias are alterations in the organization of the layers of the brain cortex due to problems in neuronal migration during development. The neuronal component has been widely studied in experimental models of cortical dysplasias. In contrast,
Cynthia Alejandra Rodríguez-Arzate, Marianne Lizeth Martínez-Mendoza, Israel Rocha-Mendoza, Yryx Luna-Palacios, Jacob Licea-Rodríguez, Ataúlfo Martínez-Torres   +5 more
doaj   +1 more source

Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams [PDF]

Scientific Reports, 2019
Abstract Light-sheet fluorescence microscopy (LSFM) has now become a unique tool in different fields ranging from three-dimensional (3D) tissue imaging to real-time functional imaging of neuronal activities. Nevertheless, obtaining high-quality artifact-free images from large, dense and inhomogeneous samples is the main challenge of ...
Kafian, Hosein, Lalenejad, Meelad, Moradi-Mehr, Sahar, Akbari Birgani, Shiva, Abdollahpour, Daryoush   +4 more
openaire   +2 more sources