Results 41 to 50 of about 38,654 (228)

Deformation Measurements of Neuronal Excitability Using Incoherent Holography Lattice Light-Sheet Microscopy (IHLLS)

Photonics, 2021
Stimuli to excitable cells and various cellular processes can cause cell surface deformations; for example, when excitable cell membrane potentials are altered during action potentials.
Mariana Potcoava, Jonathan Art, Simon Alford, Christopher Mann   +3 more
doaj   +1 more source

Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams [PDF]

Scientific Reports, 2019
Abstract Light-sheet fluorescence microscopy (LSFM) has now become a unique tool in different fields ranging from three-dimensional (3D) tissue imaging to real-time functional imaging of neuronal activities. Nevertheless, obtaining high-quality artifact-free images from large, dense and inhomogeneous samples is the main challenge of ...
Kafian, Hosein, Lalenejad, Meelad, Moradi-Mehr, Sahar, Akbari Birgani, Shiva, Abdollahpour, Daryoush   +4 more
openaire   +2 more sources

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Morphological and Calcium Signaling Alterations of Neuroglial Cells in Cerebellar Cortical Dysplasia Induced by Carmustine

Cells, 2021
Cortical dysplasias are alterations in the organization of the layers of the brain cortex due to problems in neuronal migration during development. The neuronal component has been widely studied in experimental models of cortical dysplasias. In contrast,
Cynthia Alejandra Rodríguez-Arzate, Marianne Lizeth Martínez-Mendoza, Israel Rocha-Mendoza, Yryx Luna-Palacios, Jacob Licea-Rodríguez, Ataúlfo Martínez-Torres   +5 more
doaj   +1 more source

Thermoresponsive Soft and Self‐Anchoring Cuff Electrode for Chronic Peripheral Interfaces

Advanced Functional Materials, EarlyView.
A novel thermoresponsive, soft and self‐anchoring (TSSA) cuff electrode utilizing shape memory polymers achieves autonomous, sutureless peripheral nerve fixation. Driven by thermally induced pre‐strain and tissue adhesion, the device self‐closes and softens at body temperature, successfully minimizing mechanical mismatch. This adaptive platform ensures
Seungjun Lee, Byungwook Park, Jonghee Eun, Sohee Kim, Namsun Chou   +4 more
wiley   +1 more source

A Systematic Study of GelMA‐Carbopol Bioinks for High‐Fidelity Extrusion 3D Bioprinting at Physiological Temperatures

Advanced Healthcare Materials, EarlyView.
Gonzalez Martinez and collaborators develop a strategy to formulate high performance GelMA‐based bioinks with low solids contents. The resulting bioinks enable 3D bioprinting at 37 °C of high‐fidelity structures with tunable mechanical properties that support high cell viability and function.
David A. González‐Martínez, Aidee V. Arizpe Tafoya, Eduardo González‐Martínez, Mabel Barreiro Carpio, Laura Daniela Serrano Andrade, Christopher T. N. Chow, Mohammadhossein Dabaghi, Jeremy A. Hirota, Jose M. Moran‐Mirabal   +8 more
wiley   +1 more source

Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. [PDF]

PLoS ONE, 2018
Screening cells for their differentiation potential requires a combination of tissue culture models and imaging methods that allow for long-term tracking of the location and function of cells.
Marie Held, Ilaria Santeramo, Bettina Wilm, Patricia Murray, Raphaël Lévy   +4 more
doaj   +1 more source

Multi-photon attenuation-compensated light-sheet fluorescence microscopy [PDF]

Scientific Reports, 2019
Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an ...
Veettikazhy, Madhu, Nylk, Jonathan, Gasparoli, Federico, Escobet-Montalbán, Adrià, Hansen, Anders Kragh, Marti, Dominik, Andersen, Peter Eskil, Dholakia, Kishan   +7 more
openaire   +5 more sources

Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential

Advanced Healthcare Materials, EarlyView.
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz, Manuel P. Kainz, Niklas Haider, Theresa Rienmüller, Kerstin Lenk, Rupert Kargl, Michele Terzano, Gerhard Sommer, Gerhard A. Holzapfel, Julia Fuchs   +9 more
wiley   +1 more source