Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting [PDF]
Journal of The Royal Society Interface, 2014 We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section.
Gao, Liang +3 more
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Removing striping artifacts in light-sheet fluorescence microscopy: a review [PDF]
Progress in Biophysics and Molecular Biology, 2022 In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods.
Ricci P. +7 more
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Frontiers in Neuroanatomy, 2021 The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli +19 more
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Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy [PDF]
EPJ Web of Conferences, 2023 Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs.
Tortora Giorgia +5 more
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Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy
Cell Reports, 2012 Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok +5 more
doaj +3 more sources
Bessel beam illumination reduces random and systematic errors in quantitative functional studies using light-sheet microscopy [PDF]
, 2018 Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a choice method to observe brain function with high frame rates at cellular resolution.
Alterini, Tommaso +8 more
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Phase-Retrieved Tomography enables imaging of a Tumor Spheroid in Mesoscopy Regime [PDF]
, 2016 Optical tomographic imaging of biological specimen bases its reliability on the combination of both accurate experimental measures and advanced computational techniques. In general, due to high scattering and absorption in most of the tissues, multi view
Daniele Ancora +6 more
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Multi-photon attenuation-compensated light-sheet fluorescence microscopy [PDF]
Scientific Reports, 2019 Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an ...
Veettikazhy, Madhu +7 more
openaire +5 more sources
Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser
Methods and Protocols, 2019 Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just ...
Diego Di Battista +4 more
doaj +1 more source
Live imaging of whole mouse embryos during gastrulation : migration analyses of epiblast and mesodermal cells [PDF]
, 2013 During gastrulation in the mouse embryo, dynamic cell movements including epiblast invagination and mesodermal layer expansion lead to the establishment of the three-layered body plan.
Ichikawa, Takehiko +6 more
core +3 more sources