Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly [PDF]
, 2012 Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells ...
Barbara Müller +5 more
core +1 more source
Evaluating performance in three-dimensional fluorescence microscopy [PDF]
, 2007 In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including ...
JOHN M. MURRAY +7 more
core +1 more source
Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope [PDF]
EPJ Web of Conferences, 2022 Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets ...
Hofmann Meike +5 more
doaj +1 more source
Light Sheet Fluorescence Microscopy
Proceeding of the Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020, 2020 In light-sheet fluorescence microscopy (LSFM) the optical axes of the image-forming microscope objective and the objective providing illumination of the specimen are at right angles to one another (Olarte et al., 2018). A sheet of light is incident on the specimen, and fluorescence is produced and hence detected only in that plane.
openaire +2 more sources
Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser
Methods and Protocols, 2019 Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just ...
Diego Di Battista +4 more
doaj +1 more source
Light-sheet Fluorescence Microscopy for the Study of the Murine Heart [PDF]
Journal of Visualized Experiments, 2018 Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain
Yichen, Ding +8 more
openaire +2 more sources
Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array [PDF]
, 2020 Parallelized fluorescence imaging has been a long-standing pursuit that can address the unmet need for a comprehensive three-dimensional (3D) visualization of dynamical biological processes with minimal photodamage.
Lai, TK +7 more
core +1 more source
Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy
Cell Reports, 2012 Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok +5 more
doaj +3 more sources
Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells. [PDF]
, 2011 Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Yury Prokazov +20 more
core +2 more sources
Frontiers in Neuroanatomy, 2021 The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli +19 more
doaj +1 more source