Results 61 to 70 of about 5,336,418 (265)
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
FEBS Open Bio, EarlyView.Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
npj ImagingSingle-molecule localization microscopy has revealed cellular architectures and molecular dynamics beyond the diffraction limit of light. However, imaging thick samples presents challenges from increased fluorescence background. Light sheet illumination,
Siyang Cheng +4 more
doaj +1 more source
Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. [PDF]
PLoS ONE, 2018 Screening cells for their differentiation potential requires a combination of tissue culture models and imaging methods that allow for long-term tracking of the location and function of cells.
Marie Held +4 more
doaj +1 more source
Light sheet adaptive optics microscope for 3D live imaging [PDF]
, 2013 Optical microscopy is still the main research tool for many biological studies. Indeed with the advent of genetic manipulation and specifically, the use of fluorescent protein expressing in animals and plants it has actually seen a renaissance in the ...
BOURGENOT, CYRIL,JULES,TUGDUAL
core
Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. [PDF]
, 2011 Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen.
Krombach Fritz +11 more
core +2 more sources
FEBS Open Bio, EarlyView.This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli +2 more
wiley +1 more source
Multi-photon attenuation-compensated light-sheet fluorescence microscopy [PDF]
Scientific Reports, 2019 Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an ...
Veettikazhy, Madhu +7 more
openaire +5 more sources
Photoswitching-Enabled Contrast Enhancement in Light Sheet Fluorescence Microscopy [PDF]
, 2017 Light sheet fluorescence microscopy enables high-resolution imaging of thick biological samples. By restricting the fluorescence excitation to a single plane, rapid wide-field image acquisition is possible with minimal sample exposure.
Tom Vettenburg +14 more
core +1 more source
Pressure‐Assisted Dimensional Transformation of 2D Hydrogenated Borophene into 0D Boron Quantum Dots
Advanced Functional Materials, EarlyView.Hydrogenated borophene (HB) is transformed into distinct boron nanostructures through a solvent‐free thermal process. Under flowing nitrogen, hydrogen escape promotes reconstruction into B2O3 nanoplatelets, whereas hydrogen‐induced pressure under sealed conditions facilitates fragmentation into boron quantum dots.
Ozden Gunes Yildiz +11 more
wiley +1 more source
Thin-sheet laser imaging microscopy for optical sectioning of thick tissues
BioTechniques, 2009 We report the development of a modular and optimized thin-sheet laser imaging microscope (TSLIM) for nondestructive optical sectioning of organisms and thick tissues such as the mouse cochlea, zebrafish brain/inner ear, and rat brain at a resolution that
Peter A. Santi +5 more
doaj +1 more source