Results 81 to 90 of about 5,336,418 (265)

Keeping Cells Alive in Microscopy

Biophysica
Light microscopy has emerged as one of the fundamental methods to analyze biological systems; novel techniques of 3D microscopy and super-resolution microscopy (SRM) with an optical resolution down to the sub-nanometer range have recently been realized ...
Herbert Schneckenburger, Christoph Cremer   +1 more
doaj   +1 more source

Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy

Frontiers in Cellular Neuroscience, 2014
The development of three dimensional cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex three ...
Emilio J Gualda, Daniel eSimão, Daniel eSimão, Catarina ePinto, Catarina ePinto, Paula M Alves, Paula M Alves, Catarina eBrito, Catarina eBrito   +8 more
doaj   +1 more source

Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

eLife, 2020
As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models.
Claudia Prahst, Parham Ashrafzadeh, Thomas Mead, Ana Figueiredo, Karen Chang, Douglas Richardson, Lakshmi Venkaraman, Mark Richards, Ana Martins Russo, Kyle Harrington, Marie Ouarné, Andreia Pena, Dong Feng Chen, Lena Claesson-Welsh, Kin-Sang Cho, Claudio A Franco, Katie Bentley   +16 more
doaj   +1 more source

Functional in vivo imaging using fluorescence lifetime light-sheet microscopy [PDF]

, 2017
Light-sheet microscopy has become an indispensable tool for fast, low phototoxicity volumetric imaging of biological samples, predominantly providing structural or analyte concentration data in its standard format.
Robert D. Knight, Ameer-Beg, Simon M.; id_orcid, Gerhard Holst, Thomas Gelot, Gelot, Thomas, Claire A. Mitchell, Huq, Tahiyat, Simon M. Ameer-Beg, Knight, Robert D.; id_orcid, Jakub Nedbal, Simon P. Poland, Tahiyat Huq, Mitchell, Claire A., Seyforth, James, James Seyforth, Holst, Gerhard, Poland, Simon P, Nedbal, Jakub   +17 more
core   +1 more source

Conjugated Porphyrin Polymer With Superior Charge Transfer for Photodynamic‐Immunomodulatory Prevention of CIED Infection in Diabetes

Advanced Healthcare Materials, EarlyView.
Under NIR irradiation, PTCPP generates ROS to eliminate MRSA biofilms and promote M1 macrophage polarization for phagocytosis. After bacterial clearance, the material is phagocytosed by macrophages, shifting their polarization to M2 phenotype via metabolic reprogramming, which synergistically enhances anti‐infection therapy and wound healing.
Honghao Ma, Jinguo Xu, Rongrong Gu, Chuanhui Dong, Te Chen, Yi Mu, Jing Zhu, Yating Wang, Wen Wang, Yingying Zhang, Hongyu Ma, Xiaochen Wang, Sheng Ye, Cheng Cheng   +13 more
wiley   +1 more source

Shadow imaging for panoptical visualization of brain tissue in vivo

Nature Communications, 2023
Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation (electron microscopy), do not have cellular ...
Yulia Dembitskaya, Andrew K. J. Boyce, Agata Idziak, Atefeh Pourkhalili Langeroudi, Misa Arizono, Jordan Girard, Guillaume Le Bourdellès, Mathieu Ducros, Marie Sato-Fitoussi, Amaia Ochoa de Amezaga, Kristell Oizel, Stephane Bancelin, Luc Mercier, Thomas Pfeiffer, Roger J. Thompson, Sun Kwang Kim, Andreas Bikfalvi, U. Valentin Nägerl   +17 more
doaj   +1 more source

Methodology for reconstructing early zebrafish development from in vivo multiphoton microscopy [PDF]

, 2012
Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in ...
A. Santos, T. Savy, N. Olivier, Luengo-Oroz, Miguel Angel, M. Ledesma-Carbayo, Bourgine, Paul, Olivier, Nicolas, D. Debarre, Savy, Thierry, Debarre, Delphine, Rubio-Guivernau, José L., P. Bourgine, Santos Lleo, Andres de, Faure, Emmanuel, Luengo Oroz, Miguel Ángel, Ledesma Carbayo, María Jesús, M. A. Luengo-Oroz, Pastor Escuredo, David, E. Faure, Duloquin, Louise, J. L. Rubio-Guivernau, N. Peyrieras, Ledesma-Carbayo, María Jesús, Santos, Andrés, E. Beaurepaire, Peyrieras, Nadine, Pastor-Escuredo, David, D. Pastor, L. Duloquin, Rubio Guivernau, José Luis, Beaurepaire, Emmanuel   +30 more
core   +1 more source

Targeting CD300a Signaling With a Phosphatidylserine‐Presenting Hydrogel Rescues Macrophage Dysfunction and Accelerates Diabetic Wound Healing

Advanced Healthcare Materials, EarlyView.
Schematic illustration of the proposed mechanism: PEG/RGD‐PSLs mimic apoptotic cells to engage PS receptors (notably CD300a), transducing an inhibitory signal that suppresses the MyD88/NF‐κB pathway, leading to global anti‐inflammatory and pro‐reparative effects.
Lele Wu, Xuetong Hao, Yang Zou, Xinglin Chen, Junyan Kong, Chunning Gu, Wenjie Ma, Zhongjun Yang, Huifen Liu, Mengmeng Liu, Xin Tong   +10 more
wiley   +1 more source

Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain

Frontiers in Neuroanatomy, 2019
Expansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience.
Daniel Normen Düring, Daniel Normen Düring, Daniel Normen Düring, Mariana Diales Rocha, Falk Dittrich, Manfred Gahr, Richard Hans Robert Hahnloser, Richard Hans Robert Hahnloser   +7 more
doaj   +1 more source

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

eLife, 2020
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral
Etai Sapoznik, Bo-Jui Chang, Jaewon Huh, Robert J Ju, Evgenia V Azarova, Theresa Pohlkamp, Erik S Welf, David Broadbent, Alexandre F Carisey, Samantha J Stehbens, Kyung-Min Lee, Arnaldo Marín, Ariella B Hanker, Jens C Schmidt, Carlos L Arteaga, Bin Yang, Yoshihiko Kobayashi, Purushothama Rao Tata, Rory Kruithoff, Konstantin Doubrovinski, Douglas P Shepherd, Alfred Millett-Sikking, Andrew G York, Kevin M Dean, Reto P Fiolka   +24 more
doaj   +1 more source