Results 81 to 90 of about 104,392 (267)

Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets [PDF]

Scientific Reports, 2016
AbstractWe demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm and high-speed, 4D volume ...
Zhao, Teng, Lau, Sze Cheung, Wang, Ying, Su, Yumian, Wang, Hao, Cheng, Aifang, Herrup, Karl, Ip, Nancy Y., Du, Shengwang, Loy, M.M.T.   +9 more
openaire   +3 more sources

Shaping of Biohybrid Functional Living Materials

Advanced Functional Materials, EarlyView.
This work demonstrates a strategy for shaping living mycelium into functional materials by directing its natural growth. Nanoparticles armor hyphae, micron‐scale particles entangle within the network, and printed hydrogel architectures steer expansion, creating defined geometries.
Sarah Schyck, Mark Ablonczy, Sourav Patranabish, Kunal Masania   +3 more
wiley   +1 more source

Beam shaping in light-sheet microscopy: an experimental analysis

JPhys Photonics
Thanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution.
Manuel Hüpfel, Gerd Ulrich Nienhaus
doaj   +1 more source

Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

eLife, 2020
As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models.
Claudia Prahst, Parham Ashrafzadeh, Thomas Mead, Ana Figueiredo, Karen Chang, Douglas Richardson, Lakshmi Venkaraman, Mark Richards, Ana Martins Russo, Kyle Harrington, Marie Ouarné, Andreia Pena, Dong Feng Chen, Lena Claesson-Welsh, Kin-Sang Cho, Claudio A Franco, Katie Bentley   +16 more
doaj   +1 more source

Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae

Scientific Reports, 2021
Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy.
Dimitrios Kapsokalyvas, Rodrigo Rosas, Rob W. A. Janssen, Jo M. Vanoevelen, Miranda Nabben, Martin Strauch, Dorit Merhof, Marc A. M. J. van Zandvoort   +7 more
doaj   +1 more source

Artifact‐Minimizing Ultrathin Transparent Electrodes Fabricated via iCVD for In Vivo Optogenetic Stimulation and Neural Signal Monitoring of Primary Visual Cortex

Advanced Functional Materials, EarlyView.
We present ultrathin flexible transparent electrodes through iCVD‐enabled molecular control of 10 nm gold films on poly(dimethylaminomethylstyrene). In vivo validation demonstrated photoelectric artifact reduction vs. opaque electrodes and preservation of natural neural dynamics.
Tae Jin Mun, Minju Kim, Chaesung Kim, Yunyoung Choi, In‐Yeop Baek, Younghak Cho, Gyeongyong Lee, Yerim Jang, Min‐Ho Nam, Wonryung Lee, Maesoon Im, Hyejeong Seong   +11 more
wiley   +1 more source

Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. [PDF]

, 2017
Imaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for imaging of chromatin within live cells. However, these methods are
Adli, Mazhar, Bandaria, Jigar, Darzacq, Xavier, Kuscu, Cem, Mir, Mustafa, Parlak, Mahmut, Qin, Peiwu, Singh, Ritambhara, Szlachta, Karol, Yildiz, Ahmet   +9 more
core   +1 more source

Mechanochromism of Glassy Polymers Enabled by a Loop‐Forming Supramolecular Mechanophore

Advanced Functional Materials, EarlyView.
The supramolecular mechanophore LOOP is covalently introduced into glassy polymers, capable of reporting force‐induced pre‐failure damage and local fracture events in polymer glasses. Under different mechanical deformation modes, including grinding, tensile stretching, and scratching, LOOP probes irreversible events in polymer glasses through a ...
Linlin Deng, Chantal Graafsma, Felix Meyenhofer, Iulia Scarlat, Luca Bertossi, Christoph Weder, Jess M. Clough   +6 more
wiley   +1 more source

Recent progress of second near-infrared (NIR-II) fluorescence microscopy in bioimaging

Frontiers in Physiology, 2023
Visualizing biological tissues in vivo at a cellular or subcellular resolution to explore molecular signaling and cell behaviors is a crucial direction for research into biological processes.
Tian Wang, Yingying Chen, Bo Wang, Mingfu Wu   +3 more
doaj   +1 more source

Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time [PDF]

, 2015
Fluorescence lifetime imaging (FLIM) combined with optical projection tomography (OPT) has the potential to map Förster resonant energy transfer (FRET) readouts in space and time in intact transparent or near transparent live organisms such as zebrafish ...
Alexandrov, Y, Andrews, N, Bugeon, L, Dallman, MJ, Frankel, P, French, PMW, Frolov, A, Kelly, DJ, Kerry, L, Kumar, S, Lockwood, N, McGinty, J, Ramel, M-C, Warren, SC   +13 more
core   +2 more sources