Results 91 to 100 of about 5,336,418 (265)

Human iPSC‐Derived Tissue‐Engineered Nigrostriatal Pathway for Chronic Restoration of Dopaminergic Axon Tracts in a Rat Parkinson's Model

Advanced Healthcare Materials, EarlyView.
Inspired by conventional cell therapy and emerging bioengineering approaches, the tissue‐engineered nigrostriatal pathway (TE‐NSP) has an anatomically‐inspired design comprising a population of human dopaminergic neurons with unidirectional, long‐projecting axon tracts.
Wisberty J. Gordián‐Vélez, Dimple Chouhan, Kevin D. Browne, James Lim, Saarang P. Karandikar, Rodrigo A. España, John E. Duda, H. Isaac Chen, D. Kacy Cullen   +8 more
wiley   +1 more source

Meeting report: First light sheet based fluorescence microscopy workshop [PDF]

Biotechnology Journal, 2010
In Jena, at the turn of the century, Richard Zsig-mondy (1865–1929), working together with thephysicist Heinrich Siedentopf, had the brilliantidea to use a light sheet to illuminate from the sidethe colloid solution they were investigating [1].From there the idea of light sheet illumination van-ished from the scientific spotlight until it was re-vived ...
Reynaud, E., Tomancák, P.
openaire   +3 more sources

Monitoring photosynthesis in individual cells of Synechocytis sp. PCC 6803 on a picosecond timescale [PDF]

, 2010
Picosecond fluorescence kinetics of wild-type (WT) and mutant cells of Synechocystis sp. PCC 6803, were studied at the ensemble level with a streak-camera and at the cell level using fluorescence-lifetime-imaging microscopy (FLIM).
S.P. Laptenok, van Amerongen, H., Laptenok, S., Amerongen, H., van, P Laptenok, S., H. van Amerongen, Krumova, Sb, Borst, J.W., Gombos, Z., Krumova, S.B., Z. Gombos, Krumova, S.K.B., B. Ughy, S.B. Krumova, Aijani, G., Ughy, B., Laptenok, S.P., G. Ajlani, W Borts, J., Ajlani, G., J.W. Borst   +20 more
core   +1 more source

Phase Engineering of Nanomaterials (PEN): Evolution, Current Challenges, and Future Opportunities

Advanced Materials, EarlyView.
This review summarizes the synthesis, phase transition, advanced characterization spanning ex situ to in situ and operando techniques, and diverse applications of phase engineering of nanomaterials (PEN). It further outlines key challenges and future opportunities, such as phase stability, architecture control, and artificial intelligence (AI)‐driven ...
Ye Chen, Zhanxi Fan, Jingjie Ge, Qiyuan He, Zhuangchai Lai, Jiawei Liu, Chaoliang Tan, Hua Zhang   +7 more
wiley   +1 more source

Sustainable Poly(Lactic Acid)/Graphene Oxide Bioelectronic Platform for Neurotransmitters Sensing and Tunable Stimulation of Astrocytes

Advanced Materials, EarlyView.
This work introduces sustainable PLA/graphene oxide bioelectronic interfaces featuring electrodes with tunable conductivity and strong electrocatalytic performance. These platforms were used to implement a new method for tuning astrocyte Ca2+ signaling and for the efficient detection of key biomarkers.
Alessandra Scidà, Giulia Cazzador, Roberta Fabbri, Matteo Calosi, Alessandro Silvestri, Andrea Candini, Fabiola Liscio, Franco Corticelli, Aikaterini Konstantoulaki, Andrea D'Iorio, Marco Caprini, Vincenzo Palermo, Monica Bertoldo, Valentina Benfenati, Chiara Zanardi, Emanuele Treossi   +15 more
wiley   +1 more source

Hybrid adaptive and computational light-sheet fluorescence microscopy

, 2018
The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and ...
Vdovin, Gleb (author), Fiolka, Reto (author), Dean Wilding, Gleb Vdovin, Soloviev, O.A. (author), Michel Verhaegen, Paolo Pozzi, Pozzi, P. (author), Oleg Soloviev, Reto Fiolka, Wilding, D. (author), Verhaegen, M.H.G. (author)   +11 more
core   +1 more source

Spatial Control of Light‐Responsive Proteins and Optogenetics Within Hydrogels via Volumetric Bioprinting

Advanced Materials, EarlyView.
Spatiotemporal control over cell behavior within bioprinted constructs remains a key challenge in biofabrication. Volumetric bioprinting using multi‐wavelength light sources permits to activate light‐sensitive intracellular proteins as well as optogenetic gene and protein expression circuits in bioprinted cells, in addition to enabling fast printing of
Davide Ribezzi, Pere Català, Gabriel Größbacher, Paulina Nuñez Bernal, Olaf Nijssen, Sammy Florczak, Wilco Nijenhuis, Nuria Crusellas‐Villorbina, Bram Nijhoff, Paul Delrot, Jos Malda, Andreas Hierholzer, Martin Fussenegger, Lukas C. Kapitein, Riccardo Levato   +14 more
wiley   +1 more source

Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

Nature Communications, 2017
Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view ...
Yicong Wu, Abhishek Kumar, Corey Smith, Evan Ardiel, Panagiotis Chandris, Ryan Christensen, Ivan Rey-Suarez, Min Guo, Harshad D. Vishwasrao, Jiji Chen, Jianyong Tang, Arpita Upadhyaya, Patrick J. La Riviere, Hari Shroff   +13 more
doaj   +1 more source

Ion Concentration and Voltage Imaging With Fluorescent Nanodiamonds

Advanced Materials, EarlyView.
We demonstrate reversible switching between the fluorescent NV0 and the dark NV+ state, in sub‐30 nm FNDs via surface oxidation and hydrogenation, respectively. In an electrochemical cell, we show that the fluorescence from hydrogenated particles enables all‐optical imaging of voltages and ion concentrations at the micrometer scale.
Patrick Voorhoeve, Alessandro Mameli, Hiroshi Abe, Takeshi Ohshima, Qiang Sun, Anita Quigley, Rob Kapsa, Nikolai Dontschuk, Philipp Reineck   +8 more
wiley   +1 more source

Three-dimensional exploration of the chicken embryo, a comparative study of light sheet and histological visualisation.

PLoS ONE
Ultramicroscopy has offered new avenues into the visualisation of tissues within animal models, providing three-dimensional visualisation through the use of light sheet fluorescence microscopy.
M W Smallridge, T E Aktepe, M J C Coppo, P K Vaz, A Diaz-Méndez, C M Murray, G Segal, J M Devlin, C A Hartley   +8 more
doaj   +1 more source