Results 111 to 120 of about 104,392 (267)

The Floor‐Ceiling‐Chip, or 2 × 2D = Pseudo‐3D—Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell‐Adhesive Protein Micropatterns

Advanced Healthcare Materials, EarlyView.
Here, we present a novel 3D cell patterning and culture platform. The “Floor‐Ceiling‐Chip” (FC‐Chip) consists of two opposing track‐etched membranes, creating a pseudo‐3D microenvironment for the cells in between. By providing the membranes with micropatterned cell‐adhesive islands of varying geometries and sizes, the FC‐Chip enables control over cell ...
Urandelger Tuvshindorj, Francesca Giacomini, Esra Güben Kaçmaz, Tim ten Brink, Lorenzo Moroni, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Pamela Habibović, Stefan Giselbrecht, Jan de Boer, Roman Truckenmüller   +10 more
wiley   +1 more source

Dual‐Peptide Nanoplatform: Mesoporous Silica Nanoparticles Functionalized With a Cell‐Penetrating Peptide and Loaded With Rationally Designed Antimicrobial Peptides for Tuberculosis Therapy

Advanced Healthcare Materials, EarlyView.
Machine learning–guided engineering of a plectasin‐derived peptide yields DC05, a potent antimycobacterial candidate. Encapsulation into tuftsin‐functionalized mesoporous silica nanoparticles enhances intracellular delivery, stability, and activity against Mycobacterium tuberculosis while maintaining low cytotoxicity and minimal hemolysis. The combined
Christian S. Carnero Canales, Cesar Augusto Roque‐Borda, Ana Carolina Cerqueira Negri, Oswaldo Ramirez Delgado, Letícia Oliveira Catarin Nunes, Flávia Aparecida Resende, Hernane Barud, Norival Alves Santos‐Filho, Saulo Santesso Garrido, Karen Cristina Oliveira, Alexandra Ivo de Medeiros, Rafael Miguel Sábio, Fernando Rogério Pavan   +12 more
wiley   +1 more source

Three‐dimensional imaging flow cytometry through light‐sheet fluorescence microscopy

Cytometry Part A, 2017
AbstractFlow cytometry is the tool of choice for high‐speed acquisition and analysis of large cell populations, with the tradeoff of lacking intracellular spatial information. Although in the last decades flow cytometry systems that can actually acquire two‐dimensional spatial information were developed, some of the limitations remained though, namely ...
Gualda Manzano, Emilio José, Martins, Gabriel G., Pereira, Hugo, Gardner, Rui, Moreno, Nuno   +4 more
openaire   +4 more sources

Engineering Extracellular Vesicle Production Through Magnetic Ion Channel Activation for Bone Regeneration

Advanced Healthcare Materials, EarlyView.
Magnetic Ion Channel Activation (MICA) enables remote stimulation of mechanosensitive ion channels using functionalised magnetic nanoparticles, enhancing extracellular vesicle (EV) biogenesis is pre‐osteoblasts. MICA desrived EVs exhibit typical nano‐vesicular characteristics but display superior pro‐osteogeneic activity, promoting mesenchymal stem ...
Afeesh Rajan Unnithan, Kenny Man, Kritika, Lee A. Gethings, Christopher J. Hughes, Alicia Keenan, Liam Heaney, Sophie C. Cox, Owen G. Davies, Alicia J. El Haj   +9 more
wiley   +1 more source

Ultrastructural characterization (morphological and topochemical) of wood pulp fibres [PDF]

, 2007
Different electron microscopy techniques including SEM (scanning electron microscopy), FE-SEM (field emission-scanning electron microscopy), TEM (transmission electron microscopy) and Immuno-gold TEM (immuno-gold transmission electron microscopy) were ...
Fernando, Dinesh
core  

Wound Geometry Determines Whether Aligned‐Fiber Scaffolds Accelerate or Impede Diabetic Wound Healing: A Biased Random Walk Analysis

Advanced Healthcare Materials, EarlyView.
Wound closure is governed by geometry‐orientation coupling: aligned fibers speed migration along their axis but hinder perpendicular advance. In vivo diabetic wound experiments with composition‐matched fibrin, combined with an anisotropic diffusion (biased random‐walk) model, quantify this trade‐off and generate a healing landscape.
Yin‐Yuan Huang, Xiangjun Peng, Yuxuan Huang, Kunkoo Kim, Chengli Li, Peilun Hu, Sean J. Wang, Rohan Avula, Chun‐Yi Yang, Pengchao Ma, Shuhui Yang, Shumeng Jiang, Guy M. Genin, Xiumei Wang   +13 more
wiley   +1 more source

Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co‐Cultures Maintains Their Function in a Hypoxic Environment

Advanced Healthcare Materials, EarlyView.
This study developed a bioprinted co‐culture system embedding rat pancreatic islets and Scenedesmus sp. microalgae spatially defined in close vicinity. Red light was found optimal to ensure microalgal photosynthesis while maintaining islet viability and functionality. A tailored co‐culture medium supported both cell types.
Finn Dani, Sarah Duin, Ashwini Rahul Akkineni, Susann Lehmann, Barbara Ludwig, Michael Kühl, Michael Gelinsky, Anja Lode   +7 more
wiley   +1 more source

Fluorescence of laser created electron-hole plasma in graphene

, 2010
We present an experimental observation of non-linear up- and down-converted optical luminescence of graphene and thin graphite subject to picosecond infrared laser pulses.
Kolesov, Roman, Pflaum, Jens, Stoehr, Rainer J., Wrachtrup, Joerg   +3 more
core   +1 more source

Controllable Dynamic Mechanical Cell Stimulation using Magnetically Actuated Artificial Cilia

Advanced Healthcare Materials, EarlyView.
This paper introduces a platform based on magnetic artificial cilia for providing controllable dynamic mechanical stimulation to single cells, suitable for investigating large cell populations and enabling live cell imaging. Proof‐of‐principle experiments show that cell morphology is strongly influenced by the artificial cilia, that cellular forces can
Roel Kooi, Tanveer Ul Islam, Oscar M.J.A. Stassen, Naomie Amsing, Jan de Boer, Jaap M.J. den Toonder   +5 more
wiley   +1 more source

Microengineered Gradient Hydrogels for Mechanobiology

Advanced Healthcare Materials, EarlyView.
Gradient hydrogels are used to mimic the mechanical heterogeneity in native tissues, offering powerful in vitro platforms to study cell‐material interactions in diverse pathophysiological contexts. Here, we present a comprehensive review of the design and experimental considerations for stiffness gradient hydrogels, discussing exemplary achievements ...
Shin Wei Chong, Deepu Ashok, Anna Waterhouse, Marcela M. M. Bilek, Daniele Vigolo   +4 more
wiley   +1 more source