In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy [PDF]
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent genetic kidney disorder. Animal preclinical studies are one of the main tools to study this disease, often through either 2D histology imaging for high-resolution analysis or CT ...
Pablo Delgado-Rodriguez +10 more
doaj +2 more sources
Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids [PDF]
Oksana Netschitailo +8 more
doaj +2 more sources
Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
doaj +1 more source
Innovations in ex vivo Light Sheet Fluorescence Microscopy [PDF]
Light Sheet Fluorescence Microscopy (LSFM) has revolutionized how optical imaging of biological specimens can be performed as this technique allows to produce 3D fluorescence images of entire samples with a high spatiotemporal resolution. In this manuscript, we aim to provide readers with an overview of the field of LSFM on ex vivo samples.
Pablo Delgado-Rodriguez +3 more
openaire +3 more sources
Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
openaire +2 more sources
Three-photon light-sheet fluorescence microscopy [PDF]
We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
openaire +5 more sources
Planar Airy beam light-sheet for two-photon microscopy [PDF]
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-
Almada, Pedro +12 more
core +4 more sources
3D Volume Rendering of Invertebrates Using Light-Sheet Fluorescence Microscopy
Light-Sheet Fluorescence Microscopy has recently emerged as the technique of choice for obtaining high quality three-dimensional (3D) images of whole organisms, with low photo-damage and fast acquisition rates.
Emilio J. Gualda
doaj +1 more source
Light-sheets and smart microscopy, an exciting future is dawning
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
doaj +1 more source
Light sheet fluorescence microscopy for neuroscience [PDF]
The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella +2 more
openaire +4 more sources

