Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging [PDF]
Frontiers in Neuroanatomy, 2021 Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns.
Zhouzhou Zhang +13 more
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3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy [PDF]
Communications Biology, 2022 An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Luca Pesce +11 more
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LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy [PDF]
npj ImagingCancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system.
Sabrina M. Lewis +9 more
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A guide to light-sheet fluorescence microscopy for multiscale imaging
Nature Methods, 2017 The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as developmental and cell biology, anatomical science, biophysics and neuroscience. Although adoption among biologists has been steady, LSFM has not displaced more traditional imaging methods despite its often-superior performance.
Jan Huisken
exaly +5 more sources
In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy [PDF]
Scientific ReportsAutosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent genetic kidney disorder. Animal preclinical studies are one of the main tools to study this disease, often through either 2D histology imaging for high-resolution analysis or CT ...
Pablo Delgado-Rodriguez +10 more
doaj +2 more sources
Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array. [PDF]
Laser Photon Rev, 2022 Cai Y +5 more
europepmc +2 more sources
Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids [PDF]
Biology OpenOksana Netschitailo +8 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Innovations in ex vivo Light Sheet Fluorescence Microscopy [PDF]
Progress in Biophysics and Molecular Biology, 2022 Light Sheet Fluorescence Microscopy (LSFM) has revolutionized how optical imaging of biological specimens can be performed as this technique allows to produce 3D fluorescence images of entire samples with a high spatiotemporal resolution. In this manuscript, we aim to provide readers with an overview of the field of LSFM on ex vivo samples.
Pablo Delgado-Rodriguez +3 more
openaire +3 more sources
Multiple airy beams light-sheet fluorescence microscopy
Frontiers in Physics, 2022 Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu +9 more
doaj +1 more source