Results 161 to 170 of about 13,884,188 (359)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Lighting for Transitions

open access: yes
How do we design urban lighting which feels unique and site specific but also gives the impression of a whole and cohesive nightscape?This thesis proposes working with the transitional space – the space where two different spaces meet – to overcome the ...
Møller, Alberte Strunge
core  

LightSketch - a Sketch-Modelling Program for Lighting Analysis

open access: yes, 2003
This paper presents a flexible, yet powerful lighting analysis tool, LightSketch. LightSketch is a sketch-based modelling program for lighting analysis. It allows the user to draw both architectural and lighting related symbols which are converted into a
J. Canny   +6 more
core  

Northern and Southern Lighting Cultures in Europe. Lighting Scenarios for the indoor living spaces

open access: yes, 2017
For the thesis semester, the authors, fuelled by their own life experience and driven by their recently acquired knowledge upon lighting design and their desire for implementation, attempted to create user centered lighting scenarios in order to generate
Spanos, Spyridon
core   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

open access: yesFEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth   +6 more
wiley   +1 more source

"Lighting design in the LED era" "Lighting for artworks"

open access: yes, 2017
Lectures about: 1) lighting design principles and how lighting has changed with the LED spread. 2) Lighting for cultural heritage: damage produced by light exposure as a function of sensitivity, light for improve artwork's perception and ...
Bellia L
core  

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

The Perception of Light: Understanding Architectural Lighting Design

open access: yes, 2015
Light is essential to life and vision; without light, nothing exists. It plays a pivotal role in the world of architectural design and is used to generate all manner of perceptions that enhance the designed environment experience.
McAuliffe, Marisha B.
core  

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

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