Results 71 to 80 of about 939,061 (246)
Renmin ZhujiangThe current code exhibits limitations in conducting stability analyses of dangerous rock with complex shapes and fails to consider the effects of multiple structural planes.
SU Guoshao +3 more
doaj +2 more sources
Finite Element Methods against Limit Equilibrium Approaches for Slope Stability Analysis [PDF]
, 2012 A number of powerful numerical models, including limit equilibrium and finite element (FE) methods, have been developed for slope stability analysis in recent decades.
Fatahi, B, Khabbaz, H, Nucifora, C
core
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Lattice Boltzmann Model for The Volume-Averaged Navier-Stokes Equations
, 2014 A numerical method, based on the discrete lattice Boltzmann equation, is presented for solving the volume-averaged Navier-Stokes equations. With a modified equilibrium distribution and an additional forcing term, the volume-averaged Navier-Stokes ...
Ouyang, Jie +2 more
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Soils and FoundationsEstimating the tunnel face earth pressure is essential for determining the limit support pressure in shield tunneling and for urban mountain tunnels in Japan.
Ryo Kawanoue +3 more
doaj +1 more source
Application of the limit equilibrium method to determine the safety factor for ornamental rocks [PDF]
REM: International Engineering JournalThe state of Espirito Santo is the most important producer and exporter of ornamental quarry stone. In its various quarries, it uses bench heights that range from 5, 20, 50 and 100 meters.
Rafael Franco e Silva +4 more
doaj +1 more source