Results 111 to 120 of about 8,892,596 (302)

3D Culture Protocol for Testing Gene Knockdown Efficiency and Cell Line Derivation

open access: yesBio-Protocol, 2018
Traditional 2D cell cultures with cells grown as monolayers on solid surface still represent the standard method in cancer research for drug testing. Cells grown in 2D cultures, however, lack relevant cell-matrix and cell-cell interactions and ignore the
Jan Strnadel   +5 more
doaj   +1 more source

Efficient line broadcasting in a d-dimensional grid

open access: yesDiscrete Applied Mathematics, 2001
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Amir Averbuch   +2 more
openaire   +3 more sources

Manuscript: Fear of Efficiency

open access: yes, 1915
Handwritten pages; first line of document: here are many people to whom of there… who are willing to change there ways; handwritten title Fear of Efficiency, not Tarbell's ...
Tarbell, Ida M.
core  

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

open access: yesFEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

High-Efficiency Transfection of Suspension Cell Lines

open access: yesBioTechniques, 2012
Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection.
Shereen Basiouni   +2 more
openaire   +3 more sources

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

open access: yesFEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana   +3 more
wiley   +1 more source

The effect of line balancing on the efficiency and effectiveness of shoe production at PT Wangta Agung

open access: yesJPPI (Jurnal Penelitian Pendidikan Indonesia)
Business competition is increasing, companies must reorganize their business strategies and tactics every day in the era of globalization. The purpose of this study was to determine the effect of Line Balencing on the efficiency and effectiveness of shoe
Hartono Subagio
doaj   +1 more source

Block-Run Run-Length Coding of Handwriting and Bilevel Graphics Based on Quadtree Segmentation

open access: yes, 1997
An efficient off-line coding scheme for handwriting and bilevel graphics based on quadtree segmentation and block-run run-length coding (RLC) is presented.
Yuen, H, Hanzo, L
core  

Comparing the paths of Hedgehog release in artificial and natural settings

open access: yesFEBS Letters, EarlyView.
Hh morphogens require solubilization factors to overcome their tight association with the plasma membrane and signal to distant cells, but the precise functions of these factors are controversial. Here, we use published in vivo observations as the most relevant evidence to test their suggested functions and discuss contradictions and gaps between in ...
Janna Puschmann, Kay Grobe
wiley   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

open access: yesMolecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce   +11 more
wiley   +1 more source

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