Results 231 to 240 of about 10,056,652 (288)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Correction: Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis. [PDF]

Nanomicro Lett
Zhao Q, Shi M, Yin C, Zhao Z, Zhang J, Wang J, Shen K, Zhang L, Tang H, Xiao Y, Zhang Y.   +10 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

From immature to mature epithelium: unveiling structural dynamics and transcriptional programs in rainbow trout intestinal barrier. [PDF]

Vet Res
Talamilla-Espinoza A, Vera-Tamargo F, Caruffo M, Hidalgo M, Ortiz D, Rodríguez L, Mandakovic D, Riveros A, Porras O, Pulgar R.   +9 more
europepmc   +1 more source

Sodium Line Formation in Arcturus

The Astrophysical Journal, 1975
openaire   +1 more source

Theoretical Insights into Ferromagnetism in Sm-Doped CeO₂ from Hybrid Functional: Synergistic Interplay between Sm and Oxygen Vacancy. [PDF]

ACS Omega
Han X, Benkraouda M, Maqsood S, Jithin D, Sadki EH, Amrane N.   +5 more
europepmc   +1 more source

Postoperative outcomes following institutional antibiotic protocol changes in complicated pediatric appendicitis. [PDF]

World J Pediatr Surg
Camacho-Cruz J, Vásquez-Hoyos P, Beltran-Echeverry ML, Bachiller-Tuta W, Tinoco Guzman NJ, Perdomo-Menjura A, Hoyos-Gómez L, Mendoza-Castillo D, Ferro-Martinez D.   +8 more
europepmc   +1 more source

Wound-induced syncytia outpace mononucleate neighbors during <i>Drosophila</i> wound repair. [PDF]

Elife
White JS, Hua J, Su JJ, Tro KJ, Ruark EM, Hutson MS, Page-McCaw A.   +6 more
europepmc   +1 more source