Results 91 to 100 of about 22,791 (274)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
AxiomsOrdinary differential equations are fundamental tools for modeling dynamic systems in science, engineering, and applied mathematics. Solving these equations accurately and efficiently is crucial, particularly in cases where analytical solutions are ...
Laure Gouba, Carine Ornela Megne Nono
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Carleman linearization of differential-algebraic equations systems
Results in Applied MathematicsCarleman linearization is a mathematical technique that transforms nonlinear dynamical systems into infinite-dimensional linear systems, enabling simplified analysis.
Marcos A. Hernández-Ortega +3 more
doaj +1 more source
Free integro-differential algebras and Groebner-Shirshov bases [PDF]
, 2014 The notion of commutative integro-differential algebra was introduced for the algebraic study of boundary problems for linear ordinary differential equations. Its noncommutative analog achieves a similar purpose for linear systems of such equations.
Guo, Li +5 more
core +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Differential System Approach to Gene Regulatory Network Modeling
AppliedMathThe system of ordinary differential equations that arises in models of genetic networks is considered. This system has both nonlinear and linear parts. As a nonlinear part, we use a piecewise linear function. This allows us to treat systems with multiple
Olga Kozlovska, Felix Sadyrbaev
doaj +1 more source