Results 121 to 130 of about 403,825 (264)

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Laser powder bed fusion of NiTi: effect of laser power and scanning speed on phase transformation temperatures. [PDF]

Sci Rep
Cebrián Carcavilla A, Zaki W.
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Dimensional Stability of Polymer and Titanium Implant Scan Bodies After Repeated Steam Sterilization: A High-Resolution 3D Metrological In Vitro Study. [PDF]

J Funct Biomater
Smojver I, Bjelica R, Reiser Y, Vuletić M, Prpić V, Gabrić D.   +5 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Accuracy of 3 Intraoral and 1 Extraoral Digital Scanning Systems for Multiple Laminate Veneer Preparations: An In Vitro Study. [PDF]

Med Sci Monit
Ahmad M, Sayed ME, Abdul HN, Basihi MA, Khalawi AM, Jokhadar HF, Muaddi MH, AlResayes SS, Alqarawi FK, Althubaitiy RO, Alshammakhy R, Alsaeed AY, Zidane B.   +12 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Human body surface area scales linearly with height and mass and is largely independent of body composition: a 3-D laser scanning and body composition study. [PDF]

Am J Physiol Regul Integr Comp Physiol
Jörg DJ, Kennedy S, Kotanko P, Heymsfield SB.   +3 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Long-Term Variability in Visual Processing versus Perceptual Stability. [PDF]

eNeuro
Paulsen LB, Masaracchia L, Fardo F, Ahrends C, Vidaurre D.   +4 more
europepmc   +1 more source